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A synthetic peptide corresponding to residues on the C-terminus of human Prealbumin
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents
This product is a recombinant rabbit monoclonal antibody.
Our Abpromise guarantee covers the use of ab75815 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2500 - 1/5000. Detects a band of approximately 16 kDa (predicted molecular weight: 16 kDa).|
|IHC-P||1/250 - 1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.|
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
Immunohistochemical analysis of 4% PFA in 0.1M PBS perfusion-fixed frozen murine neural tissue, labelling prealbumin with ab75815 at a dilution of 1/200 incubated for 24 hours at 4°C in 0.1M PBST with 10% donkey serum. Peermeablization was with 0.1M PBS with 3% Triton X. Blocking was with 10% serum incubated for 1 hour at 24°C. Secondary was a rabbit Alexa Fluor® 488 (green) conjugate at 1/1000. Counterstaining (red) was against GFAP with a 588 fluorescence conujugate.
Flow Cytometry analysis of HepG2 cells labelling Prealbumin with ab75815 at a dilution of 1/50 (red). Cells were fixed with 2% paraformaldehyde. A FITC-conjugated goat anti-rabbit IgG (1/150) was used as the secondary antibody. Green - Isotype control, rabbit monoclonal IgG.
ab75815 has not yet been referenced specifically in any publications.