Anti-Prealbumin 抗体 [EP2929Y] (ab75815)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP2929Y] to Prealbumin
- Suitable for: IP, Flow Cyt (Intra), WB, IHC-P
- Reacts with: Human
Related conjugates and formulations
製品の概要
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製品名
Anti-Prealbumin antibody [EP2929Y]
Prealbumin 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EP2929Y] to Prealbumin -
由来種
Rabbit -
アプリケーション
適用あり: IP, Flow Cyt (Intra), WB, IHC-Pmore details -
種交差性
交差種: Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ・コントロール
- Human heart, placenta and brain lysates; human liver tissue. IP: Human heart lysate.
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
バッファー
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.5% BSA -
Concentration information loading...
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精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EP2929Y -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab75815の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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IP |
1/50.
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Flow Cyt (Intra) |
1/50.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
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WB | (2) |
1/2500 - 1/5000. Detects a band of approximately 16 kDa (predicted molecular weight: 16 kDa).
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IHC-P | (2) |
1/250 - 1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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特記事項 |
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IP
1/50. |
Flow Cyt (Intra)
1/50. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
WB
1/2500 - 1/5000. Detects a band of approximately 16 kDa (predicted molecular weight: 16 kDa). |
IHC-P
1/250 - 1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
ターゲット情報
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機能
Thyroid hormone-binding protein. Probably transports thyroxine from the bloodstream to the brain. -
組織特異性
Detected in serum and cerebrospinal fluid (at protein level). Highly expressed in choroid plexus epithelial cells. Detected in retina pigment epithelium and liver. -
関連疾患
Defects in TTR are the cause of amyloidosis transthyretin-related (AMYL-TTR) [MIM:105210]. A hereditary generalized amyloidosis due to transthyretin amyloid deposition. Protein fibrils can form in different tissues leading to amyloid polyneuropathies, amyloidotic cardiomyopathy, carpal tunnel syndrome, systemic senile amyloidosis. The disease includes leptomeningeal amyloidosis that is characterized by primary involvement of the central nervous system. Neuropathologic examination shows amyloid in the walls of leptomeningeal vessels, in pia arachnoid, and subpial deposits. Some patients also develop vitreous amyloid deposition that leads to visual impairment (oculoleptomeningeal amyloidosis). Clinical features include seizures, stroke-like episodes, dementia, psychomotor deterioration, variable amyloid deposition in the vitreous humor.
Defects in TTR are a cause of hyperthyroxinemia dystransthyretinemic euthyroidal (HTDE) [MIM:145680]. It is a condition characterized by elevation of total and free thyroxine in healthy, euthyroid persons without detectable binding protein abnormalities.
Defects in TTR are a cause of carpal tunnel syndrome type 1 (CTS1) [MIM:115430]. It is a condition characterized by entrapment of the median nerve within the carpal tunnel. Symptoms include burning pain and paresthesias involving the ventral surface of the hand and fingers which may radiate proximally. Impairment of sensation in the distribution of the median nerve and thenar muscle atrophy may occur. This condition may be associated with repetitive occupational trauma, wrist injuries, amyloid neuropathies, rheumatoid arthritis. -
配列類似性
Belongs to the transthyretin family. -
ドメイン
Each monomer has two 4-stranded beta sheets and the shape of a prolate ellipsoid. Antiparallel beta-sheet interactions link monomers into dimers. A short loop from each monomer forms the main dimer-dimer interaction. These two pairs of loops separate the opposed, convex beta-sheets of the dimers to form an internal channel. -
細胞内局在
Secreted. Cytoplasm. - Information by UniProt
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参照データベース
- Entrez Gene: 7276 Human
- Omim: 176300 Human
- SwissProt: P02766 Human
- Unigene: 427202 Human
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別名
- Amyloid polyneuropathy antibody
- Amyloidosis I antibody
- ATTR antibody
see all
画像
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All lanes : Anti-Prealbumin antibody [EP2929Y] (ab75815) at 1/5000 dilution
Lane 1 : human heart lysate
Lane 2 : human placenta lysate
Lane 3 : human brain lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : goat anti-rabbit HRP at 1/2000 dilution
Predicted band size: 16 kDa
Observed band size: 16 kDa -
ab75815 at 1/250 dilution staining Prealbumin in human liver by Immunohistochemistry, Paraffin-embedded tissue.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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Purified ab75815 at 1/50 dilution (2µg) immunoprecipitating Prealbumin in Human heart lysate.
Lane 1 (input): Human heart lysate 10µg
Lane 2 (+): ab75815 + Human heart lysate.
Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab75815 in Human heart lysate.
VeriBlot for IP Detection Reagent (HRP) (ab131366) (1/1000 dilution) was used for Western blotting.
Blocking Buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM/TBST.
Observed band size: 16 kDa -
Intracellular Flow Cytometry analysis of HepG2 cells labelling Prealbumin with ab75815 at a dilution of 1/50 (red). Cells were fixed with 2% paraformaldehyde. A FITC-conjugated goat anti-rabbit IgG (1/150) was used as the secondary antibody. Green - Isotype control, rabbit monoclonal IgG.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (3)
ab75815 は 3 報の論文で使用されています。
- Cheng W et al. Growth differentiation factor 1-induced tumour plasticity provides a therapeutic window for immunotherapy in hepatocellular carcinoma. Nat Commun 12:7142 (2021). PubMed: 34880251
- Keeratichamroen S et al. Identification of potential cervical cancer serum biomarkers in Thai patients. Oncol Lett 19:3815-3826 (2020). PubMed: 32391095
- Katsuda T et al. Generation of human hepatic progenitor cells with regenerative and metabolic capacities from primary hepatocytes. Elife 8:N/A (2019). PubMed: 31393263