製品の概要

  • 製品名
  • 製品の詳細
    Sheep polyclonal to Prealbumin
  • アプリケーション
    適用あり: WB, IHC-P, Other, RID, Immunoelectrophoresis, Double Immunodiffusion, ICC/IFmore details
  • 種交差性
    交差種: Mouse, Rat, Rabbit, Goat, Horse, Chicken, Guinea pig, Cow, Cat, Dog, Human, Pig
  • 免疫原

    Human prealbumin purified from plasma.

  • ポジティブ・コントロール
    • This antibody gave a positive result when used in the following methanol fixed cell lines: HepG2.

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • バッファー
    Glycine buffered saline pH7.4, 0.1% Sodium Azide, 0.1% EACA, 0.01% Benzamidine,1mM EDTA
  • Concentration information loading...
  • 精製度
    IgG fraction
  • 特記事項(精製)
    Antiserum is prepared by immunisation of sheep with Human Prealbumin and, if necessary, adsorbed to monospecificity by use of solid-phase adsorbents. An immunoglobulin fraction is then produced. The titre is adjusted so that inter-batch variation is within 10%. The product is 0.2µm filtered.
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 軽鎖の種類
    unknown
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab9015 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/5000. Predicted molecular weight: 15 kDa. PubMed: 16705188
IHC-P 1/1000. PubMed: 16705188
Other Use at an assay dependent concentration.
RID Use at an assay dependent concentration.
Immunoelectrophoresis Use at an assay dependent concentration.
Double Immunodiffusion Use at an assay dependent concentration.
ICC/IF Use a concentration of 5 µg/ml.

ターゲット情報

  • 機能
    Thyroid hormone-binding protein. Probably transports thyroxine from the bloodstream to the brain.
  • 組織特異性
    Detected in serum and cerebrospinal fluid (at protein level). Highly expressed in choroid plexus epithelial cells. Detected in retina pigment epithelium and liver.
  • 関連疾患
    Defects in TTR are the cause of amyloidosis transthyretin-related (AMYL-TTR) [MIM:105210]. A hereditary generalized amyloidosis due to transthyretin amyloid deposition. Protein fibrils can form in different tissues leading to amyloid polyneuropathies, amyloidotic cardiomyopathy, carpal tunnel syndrome, systemic senile amyloidosis. The disease includes leptomeningeal amyloidosis that is characterized by primary involvement of the central nervous system. Neuropathologic examination shows amyloid in the walls of leptomeningeal vessels, in pia arachnoid, and subpial deposits. Some patients also develop vitreous amyloid deposition that leads to visual impairment (oculoleptomeningeal amyloidosis). Clinical features include seizures, stroke-like episodes, dementia, psychomotor deterioration, variable amyloid deposition in the vitreous humor.
    Defects in TTR are a cause of hyperthyroxinemia dystransthyretinemic euthyroidal (HTDE) [MIM:145680]. It is a condition characterized by elevation of total and free thyroxine in healthy, euthyroid persons without detectable binding protein abnormalities.
    Defects in TTR are a cause of carpal tunnel syndrome type 1 (CTS1) [MIM:115430]. It is a condition characterized by entrapment of the median nerve within the carpal tunnel. Symptoms include burning pain and paresthesias involving the ventral surface of the hand and fingers which may radiate proximally. Impairment of sensation in the distribution of the median nerve and thenar muscle atrophy may occur. This condition may be associated with repetitive occupational trauma, wrist injuries, amyloid neuropathies, rheumatoid arthritis.
  • 配列類似性
    Belongs to the transthyretin family.
  • ドメイン
    Each monomer has two 4-stranded beta sheets and the shape of a prolate ellipsoid. Antiparallel beta-sheet interactions link monomers into dimers. A short loop from each monomer forms the main dimer-dimer interaction. These two pairs of loops separate the opposed, convex beta-sheets of the dimers to form an internal channel.
  • 細胞内局在
    Secreted. Cytoplasm.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Amyloid polyneuropathy antibody
    • Amyloidosis I antibody
    • ATTR antibody
    • Carpal tunnel syndrome 1 antibody
    • CTS antibody
    • CTS1 antibody
    • Dysprealbuminemic euthyroidal hyperthyroxinemia antibody
    • Dystransthyretinemic hyperthyroxinemia antibody
    • Epididymis luminal protein 111 antibody
    • HEL111 antibody
    • HsT2651 antibody
    • PALB antibody
    • Prealbumin amyloidosis type I antibody
    • Prealbumin antibody
    • Prealbumin Thyroxine-binding antibody
    • Senile systemic amyloidosis antibody
    • TBPA antibody
    • Thyroxine binding prealbumin antibody
    • Transthyretin antibody
    • TTHY_HUMAN antibody
    • TTR antibody
    • TTR protein antibody
    see all

Anti-Prealbumin antibody 画像

  • ICC/IF image of ab9015 stained HepG2 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal donkey serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab9015 at 5µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 donkey anti- sheep (ab96939) IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-Prealbumin antibody (ab9015) 使用論文

This product has been referenced in:
  • Teixeira CA  et al. Impairment of autophagy by TTR V30M aggregates: in vivo reversal by TUDCA and curcumin. Clin Sci (Lond) 130:1665-75 (2016). Read more (PubMed: 27382986) »
  • Guo Z  et al. Differential urinary glycoproteome analysis of type 2 diabetic nephropathy using 2D-LC-MS/MS and iTRAQ quantification. J Transl Med 13:371 (2015). WB . Read more (PubMed: 26608305) »

See all 10 Publications for this product

Product Wall

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 27°C
Antigen retrieval step
None
Sample
Human Tissue sections (cartilage)
Specification
cartilage
Permeabilization
No
Fixative
zinc buffered formalin
Username

Abcam user community

Verified customer

投稿 Aug 20 2014

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 27°C
Antigen retrieval step
None
Sample
Mouse Tissue sections (Meniscus)
Specification
Meniscus
Permeabilization
No
Fixative
zinc buffered formalin
Username

Abcam user community

Verified customer

投稿 Aug 08 2014

Thank you for your new contact.

It has not been specifically tested on frozen sections. However, since it is suitable for formalin-fixed paraffin embedded tissues, it is very likely that it works on frozen sections as well.

If you n...

Read More

Thank you for your enquiry and your interest in our products.

I can confirm that ab9015 has been tested and characterized in IHC-P, RID, Immunoelectrophoresis, Double Immunodiffusion, WB and it recognizes pig (porcine) Prealbumin.

I...

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (human blood serum)
Loading amount
78 µg
Specification
human blood serum
Gel Running Conditions
Reduced Denaturing (15%, 6V, 45 min.)
Blocking step
Milk as blocking agent for 12 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Username

Abcam user community

Verified customer

投稿 Mar 10 2010

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Mouse Tissue lysate - other (serum)
Specification
serum
Gel Running Conditions
Reduced Denaturing
Blocking step
Milk as blocking agent for 1 hour(s) and 30 minute(s) · Concentration: 5% · Temperature: 21°C
Username

Abcam user community

Verified customer

投稿 Nov 30 2007

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Rat Cell lysate - other (serum)
Loading amount
40 µg
Specification
serum
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5%
Username

Abcam user community

Verified customer

投稿 Apr 11 2006

The antibody has not been tested in western blotting yet. According to SwissProt the predicted MW of prealbumin is around 15kDa. I hope this information helps,

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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