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Human prealbumin purified from plasma.
Our Abpromise guarantee covers the use of ab9015 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/5000. Predicted molecular weight: 15 kDa. PubMed: 16705188|
|IHC-P||1/1000. PubMed: 16705188|
|Other||Use at an assay dependent concentration.|
|RID||Use at an assay dependent concentration.|
|Immunoelectrophoresis||Use at an assay dependent concentration.|
|Double Immunodiffusion||Use at an assay dependent concentration.|
|ICC/IF||Use a concentration of 5 µg/ml.|
ICC/IF image of ab9015 stained HepG2 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal donkey serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab9015 at 5µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 donkey anti- sheep (ab96939) IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.