Anti-Prealbumin 抗体 [10E1] (ab53422)

製品の概要

  • 製品名Anti-Prealbumin antibody [10E1]
    Prealbumin 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [10E1] to Prealbumin
  • アプリケーション適用あり: WB, IP, Flow Cytmore details
    適用なし: ELISA
  • 種交差性
    交差種: Human
    交差が予測される動物種: Chimpanzee, Macaque Monkey
  • 免疫原

    Full length native protein (purified) (Human) from plasma

  • ポジティブ・コントロール
    • Human plasma

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab53422 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/2000. Detects a band of approximately 16 kDa (predicted molecular weight: 16 kDa).
IP Use at an assay dependent concentration. Recommended to be used at 1µg/400µl of lysate.
Flow Cyt Use 1µg for 106 cells. ab170191-Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.
  • 追加情報Is unsuitable for ELISA.
  • ターゲット情報

    • 機能Thyroid hormone-binding protein. Probably transports thyroxine from the bloodstream to the brain.
    • 組織特異性Detected in serum and cerebrospinal fluid (at protein level). Highly expressed in choroid plexus epithelial cells. Detected in retina pigment epithelium and liver.
    • 関連疾患Defects in TTR are the cause of amyloidosis transthyretin-related (AMYL-TTR) [MIM:105210]. A hereditary generalized amyloidosis due to transthyretin amyloid deposition. Protein fibrils can form in different tissues leading to amyloid polyneuropathies, amyloidotic cardiomyopathy, carpal tunnel syndrome, systemic senile amyloidosis. The disease includes leptomeningeal amyloidosis that is characterized by primary involvement of the central nervous system. Neuropathologic examination shows amyloid in the walls of leptomeningeal vessels, in pia arachnoid, and subpial deposits. Some patients also develop vitreous amyloid deposition that leads to visual impairment (oculoleptomeningeal amyloidosis). Clinical features include seizures, stroke-like episodes, dementia, psychomotor deterioration, variable amyloid deposition in the vitreous humor.
      Defects in TTR are a cause of hyperthyroxinemia dystransthyretinemic euthyroidal (HTDE) [MIM:145680]. It is a condition characterized by elevation of total and free thyroxine in healthy, euthyroid persons without detectable binding protein abnormalities.
      Defects in TTR are a cause of carpal tunnel syndrome type 1 (CTS1) [MIM:115430]. It is a condition characterized by entrapment of the median nerve within the carpal tunnel. Symptoms include burning pain and paresthesias involving the ventral surface of the hand and fingers which may radiate proximally. Impairment of sensation in the distribution of the median nerve and thenar muscle atrophy may occur. This condition may be associated with repetitive occupational trauma, wrist injuries, amyloid neuropathies, rheumatoid arthritis.
    • 配列類似性Belongs to the transthyretin family.
    • ドメインEach monomer has two 4-stranded beta sheets and the shape of a prolate ellipsoid. Antiparallel beta-sheet interactions link monomers into dimers. A short loop from each monomer forms the main dimer-dimer interaction. These two pairs of loops separate the opposed, convex beta-sheets of the dimers to form an internal channel.
    • 細胞内局在Secreted. Cytoplasm.
    • Information by UniProt
    • 参照データベース
    • 別名
      • Amyloid polyneuropathy antibody
      • Amyloidosis I antibody
      • ATTR antibody
      • Carpal tunnel syndrome 1 antibody
      • CTS antibody
      • CTS1 antibody
      • Dysprealbuminemic euthyroidal hyperthyroxinemia antibody
      • Dystransthyretinemic hyperthyroxinemia antibody
      • Epididymis luminal protein 111 antibody
      • HEL111 antibody
      • HsT2651 antibody
      • PALB antibody
      • Prealbumin amyloidosis type I antibody
      • Prealbumin antibody
      • Prealbumin Thyroxine-binding antibody
      • Senile systemic amyloidosis antibody
      • TBPA antibody
      • Thyroxine binding prealbumin antibody
      • Transthyretin antibody
      • TTHY_HUMAN antibody
      • TTR antibody
      • TTR protein antibody
      see all

    Anti-Prealbumin antibody [10E1] 画像

    • Overlay histogram showing JEG3 cells stained with ab53422 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab53422, 1μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H&L) (ab150113) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.
    • All lanes : Anti-Prealbumin antibody [10E1] (ab53422) at 1/2000 dilution

      Lane 1 : Transthyretin isolated from human plasma
      Lane 2 : Human plasma


      Predicted band size : 16 kDa
      Observed band size : 16 kDa
      Additional bands at : 35 kDa (possible non-specific binding),40 kDa (possible non-specific binding).

    Anti-Prealbumin antibody [10E1] (ab53422) 使用論文

    ab53422 has not yet been referenced specifically in any publications.

    Product Wall

    Application Immunoprecipitation
    Sample Human Cell lysate - other (human serum)
    Total protein in input 500 µg
    Immuno-precipitation step Other - Protein G Dynabeads
    Specification human serum
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    投稿 Jun 18 2016

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"