製品の概要

  • 製品名Anti-PRE7 antibody
  • 製品の詳細
    Mouse polyclonal to PRE7
  • アプリケーション適用あり: WBmore details
  • 種交差性
    交差種: Saccharomyces cerevisiae
  • 免疫原

    Vector coding for a partial recombinant fusion protein, corresponding to amino acids 11-110 of Saccharomyces cerevisiae PRE7. Target sequence used to make the antibody:- YSFDPVGSYEREQCRAGGAAASLIMPFLDNQVNFKNQYEPGTNGKVKK PLKYLSVEEVIKLVRDSFTSATERHIQVGDGL EILIVTKDGVRKEFYE LKRD

  • 特記事項


    This antibody was raised by a genetic immunization technique. Genetic immunization can be used to generate antibodies by directly delivering antigen-coding DNA into the animal, rather than injecting a protein or peptide (Tang et al. PubMed: 1545867; Chambers and Johnston PubMed 12910245; Barry and Johnston PubMed: 9234514). The animal's cells produce the protein, which stimulates the animal's immune system to produce antibodies against that particular protein. A vector coding for a partial fusion protein was used for genetic immunisation of a mouse and the resulting serum was tested in Western blot against an E.coli lysate containing that partial fusion protein. Genetic immunization offers enormous advantages over the traditional protein-based immunization method. DNA is faster, cheaper and easier to produce and can be produced by standard techniques readily amenable to automation. Furthermore, the antibodies generated by genetic immunization are usually of superior quality with regard to specificity, affinity and recognizing the native protein.

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • バッファーPreservative: None
    Constituents: 50% Glycerol, Whole serum
  • 精製度Whole antiserum
  • 一次抗体 備考This antibody was raised by a genetic immunization technique. Genetic immunization can be used to generate antibodies by directly delivering antigen-coding DNA into the animal, rather than injecting a protein or peptide (Tang et al. PubMed: 1545867; Chambers and Johnston PubMed 12910245; Barry and Johnston PubMed: 9234514). The animal's cells produce the protein, which stimulates the animal's immune system to produce antibodies against that particular protein. A vector coding for a partial fusion protein was used for genetic immunisation of a mouse and the resulting serum was tested in Western blot against an E.coli lysate containing that partial fusion protein. Genetic immunization offers enormous advantages over the traditional protein-based immunization method. DNA is faster, cheaper and easier to produce and can be produced by standard techniques readily amenable to automation. Furthermore, the antibodies generated by genetic immunization are usually of superior quality with regard to specificity, affinity and recognizing the native protein.
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab43700 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/1000. Predicted molecular weight: 27 kDa.

This antibody has been tested in Western blot against an E.coli lysate containing the partial recombinant fusion protein used as an immunogen. We have no data on detection of endogenous protein.

ターゲット情報

  • 関連性PRE7 is a subunit of the Yeast proteasome. The yeast proteasome seems to be composed of 14 different subunits which form a highly ordered ring-shaped structure. The proteasome degrades poly-ubiquitinated proteins in the cytoplasm and in the nucleus. It is essential for the regulated turnover of proteins and for the removal of misfolded proteins.
  • 細胞内局在Cytoplasmic and Nuclear
  • 参照データベース
    • 別名
      • PRS3 antibody
      • 20S proteasome beta type subunit antibody
      • Multicatalytic endopeptidase complex subunit C5 antibody
      • Proteasome component C5 antibody
      • PTS1 antibody
      see all

    Anti-PRE7 antibody (ab43700) 使用論文

    ab43700 has not yet been referenced specifically in any publications.

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