製品の概要

  • 製品名Anti-PRDM1/Blimp1 antibody - ChIP Grade
    PRDM1/Blimp1 一次抗体 製品一覧
  • 製品の詳細
    Goat polyclonal to PRDM1/Blimp1 - ChIP Grade
  • アプリケーション適用あり: WB, ChIP, IHC-Pmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat, Cow, Dog, Pig
  • 免疫原

    Synthetic peptide:

    C-DISDNADRLEDVED

    , corresponding to internal sequence amino acids 734-747 of Human PRDM1/Blimp1 (NP_001189.2)

  • ポジティブ・コントロール
    • A431 cell lysate

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab106766 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 0.5 - 1.5 µg/ml. Detects a band of approximately 90 kDa (predicted molecular weight: 92 kDa).
ChIP Use at an assay dependent concentration. PubMed: 23298838
IHC-P Use a concentration of 3 - 5 µg/ml.

ターゲット情報

  • 機能Transcriptional repressor that binds specifically to the PRDI element in the promoter of the beta-interferon gene (PubMed:1851123). Drives the maturation of B-lymphocytes into Ig secreting cells (PubMed:12626569).
  • 配列類似性Belongs to the class V-like SAM-binding methyltransferase superfamily.
    Contains 4 C2H2-type zinc fingers.
    Contains 1 SET domain.
  • 翻訳後修飾Sumoylation at Lys-816 by PIAS1 augments transcriptional repressor activity, and is critical for plasma cell differentiation.
    Ubiquitinated by the SCF(FBXO11) complex, leading to its degradation by the proteasome.
  • 細胞内局在Nucleus. Cytoplasm.
  • Information by UniProt
  • 参照データベース
  • 別名
    • B Lymphocyte Induced Maturation Protein 1 antibody
    • Beta interferon gene positive regulatory domain I binding factor antibody
    • Beta-interferon gene positive regulatory domain I-binding factor antibody
    • BLIMP-1 antibody
    • BLIMP1 antibody
    • Positive Regulatory Domain I Binding Factor 1 antibody
    • Positive regulatory domain I-binding factor 1 antibody
    • PR Domain Containing 1 antibody
    • PR domain containing 1 with ZNF domain antibody
    • PR domain containing 1 with ZNF domain isoform 2 antibody
    • PR domain containing protein 1 antibody
    • PR domain zinc finger protein 1 antibody
    • PR domain-containing protein 1 antibody
    • PRDI BF1 antibody
    • PRDI binding factor 1 antibody
    • PRDI-BF1 antibody
    • PRDI-binding factor 1 antibody
    • PRDM 1 antibody
    • Prdm1 antibody
    • PRDM1_HUMAN antibody
    see all

Anti-PRDM1/Blimp1 antibody - ChIP Grade 画像

  • Anti-PRDM1/Blimp1 antibody - ChIP Grade (ab106766) at 0.3 µg/ml + A431 lysate in RIPA buffer at 35 µg
    Developed using the ECL technique

    Predicted band size : 92 kDa
    Observed band size : 90 kDa (why is the actual band size different from the predicted?)


    Exposure time : 1 hour
  • Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human tonsil tissue, staining PRDM1/Blimp1 with ab106766 at 3.8 µg/ml.

Anti-PRDM1/Blimp1 antibody - ChIP Grade (ab106766) 使用論文

This product has been referenced in:
  • Kim SJ  et al. Regulation of dendritic cell activation by microRNA let-7c and BLIMP1. J Clin Invest N/A:N/A (2013). ChIP ; Mouse . Read more (PubMed: 23298838) »

See 1 Publication for this product

Product Wall

There are currently no Abreviews or Questions for ab106766.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"