製品の概要

  • 製品名
    Anti-PRDM1/Blimp1 antibody
    PRDM1/Blimp1 一次抗体 製品一覧
  • 製品の詳細
    Goat polyclonal to PRDM1/Blimp1
  • アプリケーション
    適用あり: WB, ChIP, IHC-Pmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat, Cow, Dog, Pig
  • 免疫原

    Synthetic peptide:

    C-DISDNADRLEDVED

    , corresponding to internal sequence amino acids 734-747 of Human PRDM1/Blimp1 (NP_001189.2)

  • ポジティブ・コントロール
    • A431 cell lysate

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab106766 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 0.5 - 1.5 µg/ml. Detects a band of approximately 90 kDa (predicted molecular weight: 92 kDa).
ChIP Use at an assay dependent concentration. PubMed: 23298838
IHC-P Use a concentration of 3 - 5 µg/ml.

ターゲット情報

  • 機能
    Transcriptional repressor that binds specifically to the PRDI element in the promoter of the beta-interferon gene (PubMed:1851123). Drives the maturation of B-lymphocytes into Ig secreting cells (PubMed:12626569).
  • 配列類似性
    Belongs to the class V-like SAM-binding methyltransferase superfamily.
    Contains 4 C2H2-type zinc fingers.
    Contains 1 SET domain.
  • 翻訳後修飾
    Sumoylation at Lys-816 by PIAS1 augments transcriptional repressor activity, and is critical for plasma cell differentiation.
    Ubiquitinated by the SCF(FBXO11) complex, leading to its degradation by the proteasome.
  • 細胞内局在
    Nucleus. Cytoplasm.
  • Information by UniProt
  • 参照データベース
  • 別名
    • B Lymphocyte Induced Maturation Protein 1 antibody
    • Beta interferon gene positive regulatory domain I binding factor antibody
    • Beta-interferon gene positive regulatory domain I-binding factor antibody
    • BLIMP-1 antibody
    • BLIMP1 antibody
    • Positive Regulatory Domain I Binding Factor 1 antibody
    • Positive regulatory domain I-binding factor 1 antibody
    • PR Domain Containing 1 antibody
    • PR domain containing 1 with ZNF domain antibody
    • PR domain containing 1 with ZNF domain isoform 2 antibody
    • PR domain containing protein 1 antibody
    • PR domain zinc finger protein 1 antibody
    • PR domain-containing protein 1 antibody
    • PRDI BF1 antibody
    • PRDI binding factor 1 antibody
    • PRDI-BF1 antibody
    • PRDI-binding factor 1 antibody
    • PRDM 1 antibody
    • Prdm1 antibody
    • PRDM1_HUMAN antibody
    see all

画像

  • Anti-PRDM1/Blimp1 antibody (ab106766) at 0.3 µg/ml + A431 lysate in RIPA buffer at 35 µg
    Developed using the ECL technique

    Predicted band size : 92 kDa
    Observed band size : 90 kDa (why is the actual band size different from the predicted?)


    Exposure time : 1 hour
  • Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human tonsil tissue, staining PRDM1/Blimp1 with ab106766 at 3.8 µg/ml.

参考文献

This product has been referenced in:
  • Kim SJ  et al. Regulation of dendritic cell activation by microRNA let-7c and BLIMP1. J Clin Invest N/A:N/A (2013). ChIP ; Mouse . Read more (PubMed: 23298838) »

See 1 Publication for this product

レビューと Q&A

Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (Testis, embryonic day 15.5)
Permeabilization
Yes - 0.1% Triton X-100 in PBS
Specification
Testis, embryonic day 15.5
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: 20°C
Fixative
Paraformaldehyde
Username

Mr. Bryan Niedenberger

Verified customer

投稿 Jul 05 2017

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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