製品の概要

  • 製品名Anti-PRC1 (phospho T481) antibody [EP1514Y]
    PRC1 一次抗体 製品一覧
  • 製品の詳細
    Rabbit monoclonal [EP1514Y] to PRC1 (phospho T481)
  • 特異性ab62366 detects PRC1 phosphorylated at Theonine 481.
  • アプリケーション適用あり: ICC/IF, WB, IHC-Pmore details
    適用なし: Flow Cyt or IP
  • 種交差性
    交差種: Human
  • 免疫原

    A phosphopeptide corresponding to residues surrounding Threonine 481 of human PRC1

  • ポジティブ・コントロール
    • HeLa cells and HeLa cell lysates.
  • 特記事項

    This product is a recombinant rabbit monoclonal antibody.

     

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • バッファーPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
  • 精製度Tissue culture supernatant
  • ポリ/モノモノクローナル
  • クローン名EP1514Y
  • アイソタイプIgG
  • 研究分野

関連製品

アプリケーション

Our Abpromise guarantee covers the use of ab62366 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF 1/100 - 1/250.
WB 1/500. Detects a band of approximately 72 kDa (predicted molecular weight: 72 kDa).
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
  • 追加情報Is unsuitable for Flow Cyt or IP.
  • ターゲット情報

    • 機能Cross-links antiparrallel microtubules at an average distance of 35 nM. Essential for controlling the spatiotemporal formation of the midzone and successful cytokinesis. Required for KIF14 localization to the central spindle and midbody.
    • 配列類似性Belongs to the MAP65/ASE1 family.
    • ドメインMicrotubule binding occurs via a basic patch in the central spectrin-like domain and requires also the unstructured C-terminal domain.
    • 翻訳後修飾Phosphorylation by CDK1 in early mitosis holds PRC1 in an inactive monomeric state, during the metaphase to anaphase transition, PRC1 is dephosphorylated, promoting interaction with KIF4A, which then translocates PRC1 along mitotic spindles to the plus ends of antiparallel interdigitating microtubules. Dephosphorylation also promotes MT-bundling activity by allowing dimerization.
    • 細胞内局在Nucleus. Cytoplasm. Cytoplasm > cytoskeleton > spindle pole. Predominantly localized to the nucleus of interphase cells. During mitosis becomes associated with the mitotic spindle poles and localizes with the cell midbody during cytokinesis.
    • Information by UniProt
    • 参照データベース
    • 別名
      • Anaphase spindle elongation 1 homolog antibody
      • ASE1 antibody
      • PRC1 antibody
      • PRC1_HUMAN antibody
      • Protein regulating cytokinesis 1 antibody
      • Protein regulator of cytokinesis 1 antibody
      see all

    Anti-PRC1 (phospho T481) antibody [EP1514Y] 画像

    • All lanes : Anti-PRC1 (phospho T481) antibody [EP1514Y] (ab62366) at 1/500 dilution

      Lane 1 : HeLa cell lysates (untreated)
      Lane 2 : HeLa cell lysates treated with aphidicolin + nocodazole

      Lysates/proteins at 10 µg per lane.

      Secondary
      goat anti-rabbit HRP labeled, at 1/2000 dilution

      Predicted band size : 72 kDa
      Observed band size : 72 kDa
    • ab62366 at 1/100 dilution staining PRC1 in HeLa cells by Immunocytochemistry.

    Anti-PRC1 (phospho T481) antibody [EP1514Y] (ab62366) 使用論文

    This product has been referenced in:
    • Cundell MJ  et al. A PP2A-B55 recognition signal controls substrate dephosphorylation kinetics during mitotic exit. J Cell Biol 214:539-54 (2016). Read more (PubMed: 27551054) »

    See 1 Publication for this product

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    There are currently no Abreviews or Questions for ab62366.
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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"