製品の概要

  • 製品名
    Anti-PRC1 antibody [6G2]
    PRC1 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [6G2] to PRC1
  • アプリケーション
    適用あり: WBmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Synthetic peptide, corresponding to amino acids 1/150 of Human PRC1

  • ポジティブ・コントロール
    • HeLa cell extract

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • バッファー
    Preservative: 0.09% Sodium Azide
    Constituents: PBS, pH 7.2
  • Concentration information loading...
  • 精製度
    Protein G purified
  • 特記事項(精製)
    ab39069 is an affinity purified IgG.
  • ポリ/モノ
    モノクローナル
  • クローン名
    6G2
  • アイソタイプ
    IgG2b
  • 軽鎖の種類
    kappa
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab39069 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 72 kDa.

ターゲット情報

  • 機能
    Cross-links antiparrallel microtubules at an average distance of 35 nM. Essential for controlling the spatiotemporal formation of the midzone and successful cytokinesis. Required for KIF14 localization to the central spindle and midbody.
  • 配列類似性
    Belongs to the MAP65/ASE1 family.
  • ドメイン
    Microtubule binding occurs via a basic patch in the central spectrin-like domain and requires also the unstructured C-terminal domain.
  • 翻訳後修飾
    Phosphorylation by CDK1 in early mitosis holds PRC1 in an inactive monomeric state, during the metaphase to anaphase transition, PRC1 is dephosphorylated, promoting interaction with KIF4A, which then translocates PRC1 along mitotic spindles to the plus ends of antiparallel interdigitating microtubules. Dephosphorylation also promotes MT-bundling activity by allowing dimerization.
  • 細胞内局在
    Nucleus. Cytoplasm. Cytoplasm > cytoskeleton > spindle pole. Predominantly localized to the nucleus of interphase cells. During mitosis becomes associated with the mitotic spindle poles and localizes with the cell midbody during cytokinesis.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Anaphase spindle elongation 1 homolog antibody
    • ASE1 antibody
    • PRC1 antibody
    • PRC1_HUMAN antibody
    • Protein regulating cytokinesis 1 antibody
    • Protein regulator of cytokinesis 1 antibody
    see all

画像

  • Anti-PRC1 antibody [6G2] (ab39069) at 1 µg/ml + Hela cell extracts

    Secondary
    goat anti-mouse secondary conjugated to HRP
    Developed using the ECL technique

    Predicted band size : 72 kDa
    Observed band size : 66 kDa (why is the actual band size different from the predicted?)

プロトコール

参考文献

ab39069 has not yet been referenced specifically in any publications.

レビューと Q&A

Application
Western blot
Loading amount
10 µg
Gel Running Conditions
Reduced Denaturing (4-20%)
Sample
Human Cell lysate - whole cell (293T)
Specification
293T
Treatment
Benzonase on lysate
Blocking step
BSA as blocking agent for 12 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 4°C
Username

Abcam user community

Verified customer

投稿 Jan 15 2015

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

登録