製品の概要

  • 製品名Anti-PRC1 antibody [16F2]
    PRC1 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [16F2] to PRC1
  • アプリケーション適用あり: WB, IP, ICC/IFmore details
  • 種交差性
    交差種: Mouse, Rat, Human
  • 免疫原

    Recombinant Human PRC1

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • バッファーPreservative: 0.05% Sodium azide
    Constituents: 0.1% BSA, 99% PBS
  • Concentration information loading...
  • 精製度Protein G purified
  • ポリ/モノモノクローナル
  • クローン名16F2
  • アイソタイプIgG2b
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab119338 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use at an assay dependent concentration. Predicted molecular weight: 72 kDa.
IP Use at an assay dependent concentration.
ICC/IF 1/100.

ターゲット情報

  • 機能Cross-links antiparrallel microtubules at an average distance of 35 nM. Essential for controlling the spatiotemporal formation of the midzone and successful cytokinesis. Required for KIF14 localization to the central spindle and midbody.
  • 配列類似性Belongs to the MAP65/ASE1 family.
  • ドメインMicrotubule binding occurs via a basic patch in the central spectrin-like domain and requires also the unstructured C-terminal domain.
  • 翻訳後修飾Phosphorylation by CDK1 in early mitosis holds PRC1 in an inactive monomeric state, during the metaphase to anaphase transition, PRC1 is dephosphorylated, promoting interaction with KIF4A, which then translocates PRC1 along mitotic spindles to the plus ends of antiparallel interdigitating microtubules. Dephosphorylation also promotes MT-bundling activity by allowing dimerization.
  • 細胞内局在Nucleus. Cytoplasm. Cytoplasm > cytoskeleton > spindle pole. Predominantly localized to the nucleus of interphase cells. During mitosis becomes associated with the mitotic spindle poles and localizes with the cell midbody during cytokinesis.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Anaphase spindle elongation 1 homolog antibody
    • ASE1 antibody
    • PRC1 antibody
    • PRC1_HUMAN antibody
    • Protein regulating cytokinesis 1 antibody
    • Protein regulator of cytokinesis 1 antibody
    see all

Anti-PRC1 antibody [16F2] 画像

  • Immunofluorescent analysis of PRC1 using PRC1 Monoclonal Antibody (16F2) (ab119338) shows staining in U251 Cells. PRC1 (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with an antibody recognizing PRC1 (ab119338) at a dilution of 1:20 over night at 4oC, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody. Images were taken at 60X magnification.

  • Immunofluorescent analysis of PRC1 using PRC1 Monoclonal Antibody (16F2) (ab119338) shows staining in Hela Cells. PRC1 (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with an antibody recognizing PRC1 (ab119338) at a dilution of 1:100 over night at 4oC, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody. Images were taken at 60X magnification.

  • Immunofluorescent analysis of PRC1 using PRC1 Monoclonal Antibody (16F2) (ab119338 ) shows staining in A2058 Cells. PRC1 (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with an antibody recognizing PRC1 (ab119338 ) at a dilution of 1:100 over night at 4 oC, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody. Images were taken at 60X magnification.

Anti-PRC1 antibody [16F2] (ab119338) 使用論文

ab119338 has not yet been referenced specifically in any publications.

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