Anti-PRAME 抗体 (ab89097)


  • 製品名Anti-PRAME antibody
    PRAME 一次抗体 製品一覧
  • 製品の詳細
    Mouse polyclonal to PRAME
  • アプリケーション適用あり: IHC-P, WBmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Full length Human PRAME (NP_006106.1, amino acids 1-509).

  • ポジティブ・コントロール
    • HeLa cell lysate, PRAME transfected 293T cell lysate.



Our Abpromise guarantee covers the use of ab89097 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P 1/40. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. See Abreview
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 58 kDa.


  • 機能Functions as a transcriptional repressor, inhibiting the signaling of retinoic acid through the retinoic acid receptors RARA, RARB and RARG. Prevents retinoic acid-induced cell proliferation arrest, differentiation and apoptosis.
  • 組織特異性Expressed in testis. Detected in samples of kidney, brain and skin.
  • 配列類似性Belongs to the PRAME family.
    Contains 4 LRR (leucine-rich) repeats.
  • 細胞内局在Nucleus. Cell membrane.
  • Information by UniProt
  • 参照データベース
  • 別名
    • 4930534P07Rik antibody
    • Cancer/testis antigen 130 antibody
    • CT130 antibody
    • MAPE antibody
    • Melanoma antigen preferentially expressed in tumors antibody
    • OIP 4 antibody
    • OIP-4 antibody
    • OIP4 antibody
    • OPA interacting protein 4 antibody
    • Opa interacting protein OIP4 antibody
    • OPA-interacting protein 4 antibody
    • PRAME antibody
    • PRAME_HUMAN antibody
    • Preferentially expressed antigen in melanoma antibody
    • Preferentially expressed antigen of melanoma antibody
    • RP23-250F8.3 antibody
    see all

Anti-PRAME antibody 画像

  • Anti-PRAME antibody (ab89097) at 1 µg/ml + HeLa cell lysate at 50 µg

    Predicted band size : 58 kDa
    Observed band size : 65 kDa (why is the actual band size different from the predicted?)
  • All lanes : Anti-PRAME antibody (ab89097) at 1 µg/ml

    Lane 1 : PRAME transfected 293T cell lysate
    Lane 2 : Non-transfected 293T cell lysate

    Lysates/proteins at 25 µg per lane.

    Predicted band size : 58 kDa
    Observed band size : 60 kDa (why is the actual band size different from the predicted?)
  • ab89097 staining PRAME in Human Melanoma tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde; antigen retrieval was by heat mediation with a Tris/EDTA buffer. Samples were incubated with primary antibody (1/40) for 1 hour 30 minutes at 28°C.

    See Abreview

Anti-PRAME antibody (ab89097) 使用論文

ab89097 has not yet been referenced specifically in any publications.

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (Melanoma tissue)
Specification Melanoma tissue
Fixative Formaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Tris/EDTA
Permeabilization No

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投稿 Aug 15 2011