製品の概要

  • 製品名Anti-PRAM1 antibody
    PRAM1 一次抗体 製品一覧
  • 製品の詳細
    Goat polyclonal to PRAM1
  • アプリケーション適用あり: WBmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Synthetic peptide:

    DFCDPLENQPLPLGR

    , corresponding to C terminal amino acids 656-670 of Human PRAM1.

  • ポジティブ・コントロール
    • Jurkat cell lysates.
  • 特記事項Principal Names - PRAM-1; PML-RARA target gene encoding an Adaptor Molecule-1 Official Gene Symbol - PRAM-1 GenBank Accession Number – NP_115528 LocusLink ID - 84106


    PRAM-1 is an adaptor protein interacting with proteins involved in an ATRA-signaling pathway

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • バッファーPreservative: 0.02% Sodium Azide
    Constituents: 0.5% BSA, 0.5mg/ml Tris, pH 7.3
  • Concentration information loading...
  • 精製度IgG fraction
  • 特記事項(精製)Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • 一次抗体 備考PRAM-1 is an adaptor protein interacting with proteins involved in an ATRA-signaling pathway
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野

関連製品

アプリケーション

Our Abpromise guarantee covers the use of ab805 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 - 3 µg/ml. Can be blocked with Human PRAM1 peptide (ab22785).

Approx 90-100kDa band seen in Jurkat cell lysates. Overnight incubation is recommended with this antibody.

Please note that although the predicted MW of PRAM-1 is 79kDa, Moog-Lutz et al., (J. Biol. Chem, Jun 2001; 276: 22375 - 22381) detected aPRAM-1 migrating with an apparent molecular mass of 97kDa and this is what we observed as well.

ターゲット情報

  • 機能May be involved in myeloid differentiation. May be involved in integrin signaling in neutrophils. Binds to PtdIns(4)P.
  • 組織特異性Expressed in peripheral blood leukocytes and bone marrow. Expressed in monocytes, and to a lesser extent in granulocytes and lymphocytes. Not expressed in non hematopoietic tissues except in lung.
  • 配列類似性Contains 1 SH3 domain.
  • ドメインThe SH3 domain binds to PtdIns(4)P.
  • 翻訳後修飾May be phosphorylated on tyrosines.
  • Information by UniProt
  • 参照データベース
  • 別名
    • PML RAR antibody
    • PML RARA regulated adaptor molecule 1 antibody
    • PML RARA target gene encoding an Adaptor Molecule 1 antibody
    • PML-RARA-regulated adapter molecule 1 antibody
    • PRAM 1 antibody
    • PRAM antibody
    • PRAM-1 antibody
    • PRAM_HUMAN antibody
    • PRAM1 antibody
    see all

Anti-PRAM1 antibody 画像

  • staining (2µg/ml) of Jurkat lysate (RIPA buffer, 30µg total protein per lane). Primary incubated for 12 hours. Detected by western blot using chemiluminescence

Anti-PRAM1 antibody (ab805) 使用論文

ab805 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"