製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to PRAK
  • アプリケーション
    適用あり: WB, ICC/IFmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat, Pig
  • 免疫原

    Recombinant fragment corresponding to a region within amino acids 197-467 of Human PRAK (AAH47284).

  • ポジティブ・コントロール
    • WB: 293T and H1299 whole cell lysates; A431, HeLaS3, HepG2, MOLT4 and Raji cell lines ICC/IF: HeLa cells

法規制情報

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • バッファー
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 10% Glycerol, 0.1M Tris, 0.1M Glycine, pH 7.0
  • Concentration information loading...
  • 精製度
    Immunogen affinity purified
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab97332 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/500 - 1/3000. Predicted molecular weight: 54 kDa.
ICC/IF 1/100 - 1/200.

ターゲット情報

  • 機能
    Mediates stress-induced small heat shock protein 27 phosphorylation.
  • 組織特異性
    Expressed ubiquitously.
  • 配列類似性
    Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family.
    Contains 1 protein kinase domain.
  • 翻訳後修飾
    Phosphorylated on Thr-182; which is the regulatory phosphorylation site and is located on the T-loop/loop 12.
  • 細胞内局在
    Cytoplasm. Nucleus. Also observed in the nucleus.
  • Information by UniProt
  • 参照データベース
  • 別名
    • MAP kinase-activated protein kinase 5 antibody
    • MAPK-activated protein kinase 5 antibody
    • MAPK5_HUMAN antibody
    • MAPKAP kinase 5 antibody
    • MAPKAPK-5 antibody
    • MAPKAPK5 antibody
    • Mitogen activated protein kinase activated protein kinase 5 antibody
    • p38-regulated/activated protein kinase antibody
    • PRAK antibody
    see all

画像

  • All lanes : Anti-PRAK antibody (ab97332) at 1/500 dilution

    Lane 1 : 293T whole cell lysate
    Lane 2 : H1299 whole cell lysate

    Lysates/proteins at 30 µg per lane.


    Predicted band size : 54 kDa
  • ab97332, at a 1/200 dilution, staining PRAK in paraformaldehyde fixed HeLa cells by Immunofluorescence.
    Lower image is merged with DNA probe.

参考文献

ab97332 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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