The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: 1/500 - 1/1000. Detects bands of approximately 34 and 36 kDa (predicted molecular weight: 34 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Removes thioester-linked fatty acyl groups from various substrates including S-palmitoyl-CoA. Has the highest S-thioesterase activity for the acyl groups palmitic and myristic acid followed by other short- and long-chain acyl substrates. However, because of structural constraints, is unable to remove palmitate from peptides or proteins.
Broadly expressed, with highest levels in skeletal muscle.
Belongs to the palmitoyl-protein thioesterase family.