製品の概要

  • 製品名
  • 製品の詳細
    Mouse polyclonal to PPT1
  • アプリケーション
    適用あり: WB, IHC-Pmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Full length Human PPT1, amino acids 1-306 (NP_000301.1)

  • ポジティブ・コントロール
    • Human liver tissue lysate, PPT1 transfected 293T cell lysate, Human salivary gland tissue.

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • バッファー
    Preservative: None
    Constituents: 1X PBS, pH 7.2
  • Concentration information loading...
  • 精製度
    Protein A purified
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab89022 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB
IHC-P
  • 追加情報
    IHC-P: Use at a concentration of 3 µg/ml. Antigen retrieval is recommended Place sample in 1X citrate buffer (pH 6.0) and microwave at 750W for 20 minutes, cool sample
    WB: Use at a concentration of 1 µg/ml. Detects a band of approximately 34 kDa (predicted molecular weight: 34 kDa).


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • ターゲット情報

    • 機能
      Removes thioester-linked fatty acyl groups such as palmitate from modified cysteine residues in proteins or peptides during lysosomal degradation. Prefers acyl chain lengths of 14 to 18 carbons.
    • 関連疾患
      Defects in PPT1 are the cause of neuronal ceroid lipofuscinosis type 1 (CLN1) [MIM:256730]. A form of neuronal ceroid lipofuscinosis with variable age at onset. Infantile, late-infantile, juvenile, and adult onset have been reported. Neuronal ceroid lipofuscinoses are progressive neurodegenerative, lysosomal storage diseases characterized by intracellular accumulation of autofluorescent liposomal material, and clinically by seizures, dementia, visual loss, and/or cerebral atrophy. The lipopigment pattern seen most often in CLN1 is referred to as granular osmiophilic deposits (GROD).
    • 配列類似性
      Belongs to the palmitoyl-protein thioesterase family.
    • 細胞内局在
      Lysosome.
    • Information by UniProt
    • 参照データベース
    • 別名
      • Ceroid palmitoyl palmitoyl protein thioesterase 1 antibody
      • CLN1 antibody
      • EC 3.1.2.22 antibody
      • INCL antibody
      • Palmitoyl protein hydrolase 1 antibody
      • Palmitoyl protein thioesterase 1 antibody
      • Palmitoyl-protein hydrolase 1 antibody
      • Palmitoyl-protein thioesterase 1 antibody
      • PPT antibody
      • PPT-1 antibody
      • PPT1 antibody
      • PPT1_HUMAN antibody
      see all

    Anti-PPT1 antibody 画像

    • Anti-PPT1 antibody (ab89022) at 1 µg/ml + Human liver tissue lysate at 50 µg

      Predicted band size : 34 kDa
      Observed band size : 34 kDa
    • All lanes : Anti-PPT1 antibody (ab89022) at 1 µg/ml

      Lane 1 : PPT1 transfected 293T cell lysate
      Lane 2 : Non-transfected lysate

      Lysates/proteins at 25 µg per lane.


      Predicted band size : 34 kDa
      Observed band size : 34 kDa
    • ab89022 at 3µg/ml staining PPT1 in Human salivary gland by Immunohistochemistry, Formalin-fixed, Paraffin-embedded tissue.

    Anti-PPT1 antibody (ab89022) 使用論文

    This product has been referenced in:
    • Wang R  et al. Identification of palmitoyl protein thioesterase 1 in human THP1 monocytes and macrophages and characterization of unique biochemical activities for this enzyme. Biochemistry 52:7559-74 (2013). Read more (PubMed: 24083319) »

    See 1 Publication for this product

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    There are currently no Abreviews or Questions for ab89022.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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