Anti-PPT1/PPT 抗体 (ab89022)
Key features and details
- Mouse polyclonal to PPT1/PPT
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-PPT1/PPT antibody
PPT1/PPT 一次抗体 製品一覧 -
製品の詳細
Mouse polyclonal to PPT1/PPT -
由来種
Mouse -
アプリケーション
適用あり: WB, IHC-Pmore details -
種交差性
交差種: Human -
免疫原
Recombinant full length protein within Human PPT1/PPT. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
Database link: NP_000301.1 -
ポジティブ・コントロール
- WB: Human liver tissue lysate, PPT1/PPT transfected HEK-293T cell lysate. IHC-P: Human salivary gland tissue.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
バッファー
pH: 7.40
Constituent: 100% PBS -
Concentration information loading...
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精製度
Protein A purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab89022の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB | ||
IHC-P |
特記事項 |
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WB: Use at a concentration of 1 µg/ml. Detects a band of approximately 34 kDa (predicted molecular weight: 34 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
ターゲット情報
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機能
Removes thioester-linked fatty acyl groups such as palmitate from modified cysteine residues in proteins or peptides during lysosomal degradation. Prefers acyl chain lengths of 14 to 18 carbons. -
関連疾患
Defects in PPT1 are the cause of neuronal ceroid lipofuscinosis type 1 (CLN1) [MIM:256730]. A form of neuronal ceroid lipofuscinosis with variable age at onset. Infantile, late-infantile, juvenile, and adult onset have been reported. Neuronal ceroid lipofuscinoses are progressive neurodegenerative, lysosomal storage diseases characterized by intracellular accumulation of autofluorescent liposomal material, and clinically by seizures, dementia, visual loss, and/or cerebral atrophy. The lipopigment pattern seen most often in CLN1 is referred to as granular osmiophilic deposits (GROD). -
配列類似性
Belongs to the palmitoyl-protein thioesterase family. -
細胞内局在
Lysosome. - Information by UniProt
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参照データベース
- Entrez Gene: 5538 Human
- Omim: 600722 Human
- SwissProt: P50897 Human
- Unigene: 3873 Human
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別名
- Ceroid palmitoyl palmitoyl protein thioesterase 1 antibody
- CLN1 antibody
- EC 3.1.2.22 antibody
see all
画像
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Anti-PPT1/PPT antibody (ab89022) at 1 µg/ml + Human liver tissue lysate at 50 µg
Predicted band size: 34 kDa
Observed band size: 34 kDa -
All lanes : Anti-PPT1/PPT antibody (ab89022) at 1 µg/ml
Lane 1 : PPT1/PPT transfected HEK-293T cell lysate
Lane 2 : Non-transfected lysate
Lysates/proteins at 25 µg per lane.
Predicted band size: 34 kDa
Observed band size: 34 kDa -
ab89022 at 3µg/ml staining PPT1/PPT in Human salivary gland by Immunohistochemistry, Formalin-fixed, Paraffin-embedded tissue.
プロトコール
データシートおよび資料
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Datasheet download
参考文献 (3)
ab89022 は 3 報の論文で使用されています。
- Sima N et al. Neural stem cells for disease modeling and evaluation of therapeutics for infantile (CLN1/PPT1) and late infantile (CLN2/TPP1) neuronal ceroid lipofuscinoses. Orphanet J Rare Dis 13:54 (2018). WB . PubMed: 29631617
- Shyng C et al. Widespread Expression of a Membrane-Tethered Version of the Soluble Lysosomal Enzyme Palmitoyl Protein Thioesterase-1. JIMD Rep 36:85-92 (2017). PubMed: 28213849
- Wang R et al. Identification of palmitoyl protein thioesterase 1 in human THP1 monocytes and macrophages and characterization of unique biochemical activities for this enzyme. Biochemistry 52:7559-74 (2013). PubMed: 24083319