Anti-PPP6C/Ppv 抗体 (ab70634)
Key features and details
- Rabbit polyclonal to PPP6C/Ppv
- Suitable for: WB, IP
- Reacts with: Mouse, Human
- Isotype: IgG
製品の概要
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製品名
Anti-PPP6C/Ppv antibody
PPP6C/Ppv 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to PPP6C/Ppv -
由来種
Rabbit -
アプリケーション
適用あり: WB, IPmore details -
種交差性
交差種: Mouse, Human
交差が予測される動物種: Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Dog, Turkey, Pig, Xenopus laevis, Chimpanzee, Zebrafish, Rhesus monkey, Gorilla, Orangutan, Xenopus tropicalis -
免疫原
Synthetic peptide corresponding to Human PPP6C/Ppv (C terminal).
Database link: NP_002712.1 -
ポジティブ・コントロール
- Whole cell lysates from HeLa, 293T and NIH3T3 cells.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 7
Preservative: 0.09% Sodium azide
Constituents: 1.764% Sodium citrate, 1.815% Tris, 0.021% PBS -
Concentration information loading...
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精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab70634の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB | (1) |
1/1000 - 1/10000. Detects a band of approximately 35 kDa (predicted molecular weight: 35 kDa).
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IP |
Use at 2-5 µg/mg of lysate.
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特記事項 |
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WB
1/1000 - 1/10000. Detects a band of approximately 35 kDa (predicted molecular weight: 35 kDa). |
IP
Use at 2-5 µg/mg of lysate. |
ターゲット情報
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機能
Catalytic subunit of protein phospatase 6 (PP6). PP6 is a component of a signaling pathway regulating cell cycle progression in response to IL2 receptor stimulation. N-terminal domain restricts G1 to S phase progression in cancer cells, in part through control of cyclin D1. Downregulates MAP3K7 kinase activation of the IL1 signaling pathway by dephosphorylation of MAP3K7. -
組織特異性
Ubiquitously expressed in all tissues tested with highest expression levels in testis, heart, kidney, brain, stomach, liver and skeletal muscle and lowest in placenta, lung colon and spleen. -
配列類似性
Belongs to the PPP phosphatase family. PP-6 (PP-V) subfamily. -
細胞内局在
Cytoplasm. - Information by UniProt
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参照データベース
- Entrez Gene: 511058 Cow
- Entrez Gene: 608922 Dog
- Entrez Gene: 5537 Human
- Entrez Gene: 67857 Mouse
- Entrez Gene: 100153256 Pig
- Entrez Gene: 171121 Rat
- Entrez Gene: 734439 Xenopus laevis
- Entrez Gene: 393980 Zebrafish
see all -
別名
- FLJ92648 antibody
- MGC12249 antibody
- PP 6 antibody
see all
画像
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All lanes : Anti-PPP6C/Ppv antibody (ab70634) at 0.4 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : 293T whole cell lysate at 50 µg
Lane 5 : NIH3T3 whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 35 kDa
Observed band size: 35 kDa
Exposure time: 3 minutes -
Detection of PPP6C/Ppv by Western Blot of Immunoprecipitate. ab70634 at 1µg/ml staining PPP6C/Ppv in HeLa whole cell lysate immunoprecipitated using ab70634 at 3µg/mg lysate (1 mg/IP; 20% of IP loaded/lane). Detection: chemiluminescence with exposure time of 30 seconds.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (1)
ab70634 は 1 報の論文で使用されています。
- Gallegos LL et al. A protein interaction map for cell-cell adhesion regulators identifies DUSP23 as a novel phosphatase for ß-catenin. Sci Rep 6:27114 (2016). PubMed: 27255161