製品の概要

  • 製品名Anti-PPP4R2 antibody
    PPP4R2 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to PPP4R2
  • アプリケーション適用あり: WB, IPmore details
  • 種交差性
    交差種: Mouse, Human
    交差が予測される動物種: Horse, Cow, Pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan
  • 免疫原

    Synthetic peptide corresponding to a region between residue 370 and the C-terminus (residue 417) of Human PPP4R2 (NP_777567.1)

  • ポジティブ・コントロール
    • Whole cell lysates from HeLa, 293T and NIH3T3 cells.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab70631 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB
IP
  • 追加情報IP: Use at 2-5µg/mg of lysate.
    WB: 1/2000 - 1/10000. Detects a band of approximately 65 kDa (predicted molecular weight: 47 kDa).


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • ターゲット情報

    Anti-PPP4R2 antibody 画像

    • All lanes : Anti-PPP4R2 antibody (ab70631) at 0.04 µg/ml

      Lane 1 : HeLa whole cell lysate at 50 µg
      Lane 2 : HeLa whole cell lysate at 15 µg
      Lane 3 : HeLa whole cell lysate at 5 µg
      Lane 4 : 293T whole cell lysate at 50 µg
      Lane 5 : NIH3T3 whole cell lysate at 50 µg

      Developed using the ECL technique

      Predicted band size : 47 kDa
      Observed band size : 65 kDa (why is the actual band size different from the predicted?)


      Exposure time : 30 seconds
    • Detection of Human PPP4R2 by Western Blot of Immunprecipitate. ab70631 at 1µg/ml staining PPP4R2 in HeLa whole cell lysate immunoprecipitated using ab70631 at 3µg/mg lysate (1 mg/IP; 20% of IP loaded/lane). Detection: chemiluminescence with exposure time of 10 seconds.

    Anti-PPP4R2 antibody (ab70631) 使用論文

    This product has been referenced in:

    See 1 Publication for this product

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