製品の概要

  • 製品名Anti-PPP4C antibody
    PPP4C 一次抗体 製品一覧
  • 製品の詳細
    Goat polyclonal to PPP4C
  • アプリケーション適用あり: WB, IHC-Pmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat, Rabbit, Cow, Dog, Xenopus laevis, Zebrafish, Orangutan
  • 免疫原

    Synthetic peptide:

    C-PQETRGIPSKKP

    , corresponding to C terminal amino acids 290-301 of Human PPP4C (NP_002711.1).

  • ポジティブ・コントロール
    • HEK293, A431 and HeLa cell lysates.

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • バッファーPreservative: 0.02% Sodium Azide
    Constituents: 0.5% BSA, Tris saline, pH 7.3
  • Concentration information loading...
  • 精製度Immunogen affinity purified
  • 特記事項(精製)ab87672 is purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab87672 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 0.1 - 0.3 µg/ml. Detects a band of approximately 34 kDa (predicted molecular weight: 34 kDa).
IHC-P Use a concentration of 2 - 4 µg/ml.

ターゲット情報

  • 機能Protein phosphatase that is involved in many processes such as microtubule organization at centrosomes, maturation of spliceosomal snRNPs, apoptosis, DNA repair, tumor necrosis factor (TNF)-alpha signaling, activation of c-Jun N-terminal kinase MAPK8, regulation of histone acetylation, DNA damage checkpoint signaling, NF-kappa-B activation and cell migration. The PPP4C-PPP4R1 PP4 complex may play a role in dephosphorylation and regulation of HDAC3. The PPP4C-PPP4R2-PPP4R3A PP4 complex specifically dephosphorylates H2AFX phosphorylated on Ser-140 (gamma-H2AFX) generated during DNA replication and required for DNA double strand break repair. Dephosphorylates NDEL1 at CDK1 phosphorylation sites and negatively regulates CDK1 activity in interphase (By similarity). In response to DNA damage, catalyzes RPA2 dephosphorylation, an essential step for DNA repair since it allows the efficient RPA2-mediated recruitment of RAD51 to chromatin.
  • 配列類似性Belongs to the PPP phosphatase family. PP-4 (PP-X) subfamily.
  • 細胞内局在Cytoplasm. Nucleus. Cytoplasm > cytoskeleton > centrosome.
  • Information by UniProt
  • 参照データベース
  • 別名
    • PP X antibody
    • PP-X antibody
    • Pp4 antibody
    • PP4C antibody
    • PP4C_HUMAN antibody
    • PPH3 antibody
    • PPP4 antibody
    • ppp4c antibody
    • PPX antibody
    • protein phosphatase 4 (formerly X), catalytic subunit antibody
    • Protein phosphatase 4 catalytic subunit antibody
    • Protein phosphatase X antibody
    • protein phosphatase X, catalytic subunit antibody
    • Serine/threonine protein phosphatase 4 catalytic subunit antibody
    • Serine/threonine-protein phosphatase 4 catalytic subunit antibody
    see all

Anti-PPP4C antibody 画像

  • Immunohistochemical staining of paraffin embedded human prostate with ab87672 at 2.5 µg/mL. Heat mediated antigen retrieval was performed with citrate buffer at pH 6.

  • All lanes : Anti-PPP4C antibody (ab87672) at 0.3 µg/ml

    Lane 1 : rat spleen tissue lysate
    Lane 2 : rat lung tissue lysate

    Lysates/proteins at 35 µg per lane.


    Predicted band size : 34 kDa
    Observed band size : 35 kDa (why is the actual band size different from the predicted?)
  • Anti-PPP4C antibody (ab87672) at 0.3 µg/ml + HEK293 lysate (in RIPA buffer) at 35 µg
    Developed using the ECL technique

    Predicted band size : 34 kDa
    Observed band size : 34 kDa
    Primary incubation was 1 hour.

Anti-PPP4C antibody (ab87672) 使用論文

ab87672 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"