製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to PPP4C
  • アプリケーション
    適用あり: ICC/IF, WB, ICCmore details
  • 種交差性
    交差種: Mouse, Rat, Cow, Human
    交差が予測される動物種: Rabbit
  • 免疫原

    Synthetic peptide:

    RGIPSKKPVADYFL

    conjugated to KLH, corresponding to amino acids 294-307 of Human PPP4C.

  • ポジティブ・コントロール
    • Bovine testes homogenate.

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • バッファー
    Preservative: 0.08% Sodium Azide
    Constituents: PBS
  • Concentration information loading...
  • 精製度
    Protein A purified
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab16475 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF 1/300.
WB 1/500 - 1/1000. Predicted molecular weight: 34 kDa.
ICC 1/500 - 1/1000.

ターゲット情報

  • 機能
    Protein phosphatase that is involved in many processes such as microtubule organization at centrosomes, maturation of spliceosomal snRNPs, apoptosis, DNA repair, tumor necrosis factor (TNF)-alpha signaling, activation of c-Jun N-terminal kinase MAPK8, regulation of histone acetylation, DNA damage checkpoint signaling, NF-kappa-B activation and cell migration. The PPP4C-PPP4R1 PP4 complex may play a role in dephosphorylation and regulation of HDAC3. The PPP4C-PPP4R2-PPP4R3A PP4 complex specifically dephosphorylates H2AFX phosphorylated on Ser-140 (gamma-H2AFX) generated during DNA replication and required for DNA double strand break repair. Dephosphorylates NDEL1 at CDK1 phosphorylation sites and negatively regulates CDK1 activity in interphase (By similarity). In response to DNA damage, catalyzes RPA2 dephosphorylation, an essential step for DNA repair since it allows the efficient RPA2-mediated recruitment of RAD51 to chromatin.
  • 配列類似性
    Belongs to the PPP phosphatase family. PP-4 (PP-X) subfamily.
  • 細胞内局在
    Cytoplasm. Nucleus. Cytoplasm > cytoskeleton > centrosome.
  • Information by UniProt
  • 参照データベース
  • 別名
    • PP X antibody
    • PP-X antibody
    • Pp4 antibody
    • PP4C antibody
    • PP4C_HUMAN antibody
    • PPH3 antibody
    • PPP4 antibody
    • ppp4c antibody
    • PPX antibody
    • protein phosphatase 4 (formerly X), catalytic subunit antibody
    • Protein phosphatase 4 catalytic subunit antibody
    • Protein phosphatase X antibody
    • protein phosphatase X, catalytic subunit antibody
    • Serine/threonine protein phosphatase 4 catalytic subunit antibody
    • Serine/threonine-protein phosphatase 4 catalytic subunit antibody
    see all

画像

  • All lanes : Anti-PPP4C antibody (ab16475) at 1/10000 dilution (in PBS + 5% milk +0.1% Tween 20 for 1 hour at 25°C)

    Lane 1 : HeLa whole cell lysate - mock transfected
    Lane 2 : HeLa whole cell lysate - transfected with human siRNA against PPP4C

    Lysates/proteins at 100000 cells per lane.

    Secondary
    An HRP-conjugated Goat anti-rabbit IgG polyclonal at 1/50000 dilution

    Performed under non-reducing conditions.

    Predicted band size : 34 kDa
    Observed band size : 34 kDa
    Additional bands at : 50 kDa. We are unsure as to the identity of these extra bands.

    This image is courtesy of an anonymous abreview.

    Blocking Step: 5% Milk for 1 hour at 25°C

    See Abreview

参考文献

This product has been referenced in:

See 1 Publication for this product

レビューと Q&A

Application
Western blot
Loading amount
10000 cells
Gel Running Conditions
Reduced Denaturing (4-12% gradient)
Sample
Human Cell lysate - whole cell (Huh7 liver)
Specification
Huh7 liver
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

投稿 Mar 31 2015

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (Hela cells)
Loading amount
100000 cells
Specification
Hela cells
Gel Running Conditions
Non-reduced Non-Denaturing (Native) (Invitrogen NuPage 4-12% Bis-Tris)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

投稿 Sep 22 2010

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (Hela)
Specification
Hela
Fixative
Methanol
Permeabilization
No
Blocking step
Fish scale gelatin as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 0.2% · Temperature: 25°C
Username

Abcam user community

Verified customer

投稿 Jul 15 2010

I think there are two potential areas which the customer should consider looking into: the protocol and the samples. I am sure the customer knows very well his area of research and trust that many papers show PPP4C where he expects it, unfortunately, ...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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