製品の概要

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • バッファー
    Preservative: 0.02% Sodium Azide
    Constituents: 0.5% BSA, 5mg/ml Tris, pH 7.3
  • Concentration information loading...
  • 精製度
    IgG fraction
  • 特記事項(精製)
    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab1083 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB
  • 追加情報
    Western Blot – Approx 55kDa and 40kDa bands seen in Mouse Brain extracts. Recommended for use at 0.5-2µg/ml.

    Please note that the two bands may correspond to two different isoforms. Two isoforms of the epsilon mRNA have been detected in Northern blots (J Biol Chem 1996 Sep 6;271(36):22081-9).
  • ターゲット情報

    • 機能
      The B regulatory subunit might modulate substrate selectivity and catalytic activity, and also might direct the localization of the catalytic enzyme to a particular subcellular compartment.
    • 配列類似性
      Belongs to the phosphatase 2A regulatory subunit B56 family.
    • 翻訳後修飾
      Phosphorylated on serine residues.
    • 細胞内局在
      Cytoplasm.
    • Information by UniProt
    • 参照データベース
    • 別名
      • 2A5E_HUMAN antibody
      • Epsilon isoform of regulatory subunit B56 protein phosphatase 2A antibody
      • PP2A B subunit B' epsilon antibody
      • PP2A B subunit B' epsilon isoform antibody
      • PP2A B subunit B56 epsilon antibody
      • PP2A B subunit B56 epsilon isoform antibody
      • PP2A B subunit isoform B''-epsilon antibody
      • PP2A B subunit isoform B'-epsilon antibody
      • PP2A B subunit isoform B56-epsilon antibody
      • PP2A B subunit isoform PR61-epsilon antibody
      • PP2A B subunit isoform R5-epsilon antibody
      • PP2A B subunit PR61 epsilon antibody
      • PP2A B subunit PR61 epsilon isoform antibody
      • PP2A B subunit R5 epsilon antibody
      • PP2A B subunit R5 epsilon isoform antibody
      • PPP2R5E antibody
      • Protein phosphatase 2 regulatory subunit B (B56) epsilon isoform antibody
      • Protein phosphatase 2 regulatory subunit B' epsilon antibody
      • Protein phosphatase 2 regulatory subunit B' epsilon isoform antibody
      • Regulatory subunit B of protein phosphatase 2 epsilon isoform antibody
      • Serine/threonine protein phosphatase 2A 56 kDa regulatory subunit epsilon antibody
      • Serine/threonine-protein phosphatase 2A 56 kDa regulatory subunit epsilon isoform antibody
      see all

    Anti-PPP2R5E antibody 画像

    • Staining (0.5µg/ml) of Mouse Brain extracts (RIPA buffer, 35µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.

    プロトコール

    Anti-PPP2R5E antibody (ab1083) 使用論文

    ab1083 has not yet been referenced specifically in any publications.

    Product Wall

    Abreviews
    Abcam has not validated the combination of species/application used in this Abreview.
    Application
    Immunocytochemistry/ Immunofluorescence
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
    Sample
    Mouse Cell (Oocyte)
    Specification
    Oocyte
    Permeabilization
    Yes - Triton X-100 0.1%
    Fixative
    Paraformaldehyde
    Username

    Abcam user community

    Verified customer

    投稿 May 29 2013

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Western blot
    Sample
    Mouse Tissue lysate - whole (Organ, 293 cell)
    Loading amount
    25 µg
    Specification
    Organ, 293 cell
    Gel Running Conditions
    Reduced Denaturing (10%)
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Username

    Abcam user community

    Verified customer

    投稿 Oct 13 2010

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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