Rabbit polyclonal to PPP2R5D
Horse, Guinea pig, Cow, Dog, Pig, Chimpanzee, Rhesus monkey, Opossum, Orangutan, Elephant
Synthetic peptide corresponding to a region between residues 1 and 50 of human PPP2R5D (NP_006236.1).
Whole cell lysate from HeLa cells.
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Preservative: 0.09% Sodium Azide
Constituents: 0.1% BSA, Tris buffered saline
Concentration information loading...
Immunogen affinity purified
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/2000 - 1/10000. Detects a band of approximately 74 kDa (predicted molecular weight: 67 kDa).
Use at 2-5 µg/mg of lysate.
The B regulatory subunit might modulate substrate selectivity and catalytic activity, and also might direct the localization of the catalytic enzyme to a particular subcellular compartment.
Isoform Delta-2 is widely expressed. Isoform Delta-1 is highly expressed in brain.
Belongs to the phosphatase 2A regulatory subunit B56 family.
Cytoplasm. Nucleus. Nuclear in interphase, nuclear during mitosis.
Information by UniProt
Western blot - PPP2R5D antibody (ab72303)
All lanes :
Anti-PPP2R5D antibody (ab72303) at 0.04 µg/ml
Lane 1 :
Whole cell lysate from HeLa cells at 50 µg
Lane 2 :
Whole cell lysate from HeLa cells at 15 µg
Lane 3 :
Whole cell lysate from HeLa cells at 5 µg
Developed using the ECL technique.
Predicted band size:
Observed band size:
74 kDa (
why is the actual band size different from the predicted?
Immunoprecipitation - PPP2R5D antibody (ab72303)
Detection of Human PPP2R5D by Immunoprecipitation in whole cell lysate from HeLa cells (1 mg for IP, 20% of IP loaded), using ab72303 at 3 µg/mg lysate for IP and at 0.1 µg/ml for subsequent Western blot detection.
Detection: Chemiluminescence with an exposure time of 10 seconds.
has not yet been referenced specifically in any publications.
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