製品の概要

  • 製品名Anti-PPP2R5D antibody
    PPP2R5D 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to PPP2R5D
  • アプリケーション適用あり: WB, IPmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Horse, Guinea pig, Cow, Dog, Pig, Chimpanzee, Rhesus monkey, Opossum, Orangutan, Elephant
  • 免疫原

    Synthetic peptide corresponding to a region between residues 1 and 50 of human PPP2R5D (NP_006236.1).

  • ポジティブ・コントロール
    • Whole cell lysate from HeLa cells.

製品の特性

関連製品

アプリケーション

Our Abpromise guarantee covers the use of ab72303 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/2000 - 1/10000. Detects a band of approximately 74 kDa (predicted molecular weight: 67 kDa).
IP Use at 2-5 µg/mg of lysate.

ターゲット情報

  • 機能The B regulatory subunit might modulate substrate selectivity and catalytic activity, and also might direct the localization of the catalytic enzyme to a particular subcellular compartment.
  • 組織特異性Isoform Delta-2 is widely expressed. Isoform Delta-1 is highly expressed in brain.
  • 配列類似性Belongs to the phosphatase 2A regulatory subunit B56 family.
  • 細胞内局在Cytoplasm. Nucleus. Nuclear in interphase, nuclear during mitosis.
  • Information by UniProt
  • 参照データベース
  • 別名
    • 2A5D_HUMAN antibody
    • B'delta antibody
    • B56D antibody
    • Delta isoform of regulatory subunit B56, protein phosphatase 2A antibody
    • MGC2134 antibody
    • MGC8949 antibody
    • OTTHUMP00000039821 antibody
    • PP2A B subunit B' delta isoform antibody
    • PP2A B subunit B56 delta isoform antibody
    • PP2A B subunit isoform B''-delta antibody
    • PP2A B subunit isoform B56-delta antibody
    • PP2A B subunit isoform PR61-delta antibody
    • PP2A B subunit isoform R5-delta antibody
    • PP2A B subunit PR61 delta isoform antibody
    • PP2A B subunit R5 delta isoform antibody
    • PPP2R5D antibody
    • Protein phosphatase 2 regulatory subunit B (B56) delta isoform antibody
    • Protein phosphatase 2 regulatory subunit B delta isoform antibody
    • Protein phosphatase 2 regulatory subunit B' delta antibody
    • Serine threonine protein phosphatase 2A 56 kDa regulatory subunit delta isoform antibody
    • Serine/threonine-protein phosphatase 2A 56 kDa regulatory subunit delta isoform antibody
    • TEG-271 antibody
    • Tex271 antibody
    see all

Anti-PPP2R5D antibody 画像

  • All lanes : Anti-PPP2R5D antibody (ab72303) at 0.04 µg/ml

    Lane 1 : Whole cell lysate from HeLa cells at 50 µg
    Lane 2 : Whole cell lysate from HeLa cells at 15 µg
    Lane 3 : Whole cell lysate from HeLa cells at 5 µg

    Developed using the ECL technique

    Predicted band size : 67 kDa
    Observed band size : 74 kDa (why is the actual band size different from the predicted?)


    Exposure time : 10 seconds
  • Detection of Human PPP2R5D by Immunoprecipitation in whole cell lysate from HeLa cells (1 mg for IP, 20% of IP loaded), using ab72303 at 3 µg/mg lysate for IP and at 0.1 µg/ml for subsequent Western blot detection.
    Detection: Chemiluminescence with an exposure time of 10 seconds. seconds

Anti-PPP2R5D antibody (ab72303) 使用論文

ab72303 has not yet been referenced specifically in any publications.

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (HeLa)
Loading amount 100 µg
Specification HeLa
Gel Running Conditions Reduced Denaturing (12% gel)
Blocking step Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: RT°C
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投稿 Sep 28 2012

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"