製品の概要

  • 製品名
  • 製品の詳細
    Mouse polyclonal to PPP2R5A
  • アプリケーション
    適用あり: WBmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Full length Human PPP2R5A (NP_006234.1)

  • ポジティブ・コントロール
    • Human placenta tissue lysate Jurkat cell lysate Transfected 293T cell lysate

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab89621 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 56 kDa.

ターゲット情報

  • 機能
    The B regulatory subunit might modulate substrate selectivity and catalytic activity, and also might direct the localization of the catalytic enzyme to a particular subcellular compartment.
  • 組織特異性
    Widely expressed with the highest expression in heart and skeletal muscle.
  • 配列類似性
    Belongs to the phosphatase 2A regulatory subunit B56 family.
  • 翻訳後修飾
    Phosphorylated on serine residues.
  • 細胞内局在
    Cytoplasm. Nucleus. Chromosome > centromere. From mitotic prophase to metaphase, localizes at the inner centromere between a pair of sister kinetochores. Decreased expression at the onset of anaphase.
  • Information by UniProt
  • 参照データベース
  • 別名
    • 2A5A_HUMAN antibody
    • B56A antibody
    • MGC131915 antibody
    • PP2A B subunit B' alpha isoform antibody
    • PP2A B subunit B56 alpha isoform antibody
    • PP2A B subunit isoform B''-alpha antibody
    • PP2A B subunit isoform B56-alpha antibody
    • PP2A B subunit isoform PR61-alpha antibody
    • PP2A B subunit isoform R5-alpha antibody
    • PP2A B subunit PR61 alpha isoform antibody
    • PP2A B subunit R5 alpha isoform antibody
    • PPP2R5A antibody
    • PR61A antibody
    • PR61alpha antibody
    • Protein phosphatase 2 regulatory subunit B (B56) alpha isoform antibody
    • Protein phosphatase 2 regulatory subunit B' alpha antibody
    • Serine/threonine-protein phosphatase 2A 56 kDa regulatory subunit alpha isoform antibody
    see all

画像

  • Anti-PPP2R5A antibody (ab89621) at 1 µg/ml + Human placenta tissue lysate at 50 µg

    Predicted band size : 56 kDa
  • Anti-PPP2R5A antibody (ab89621) at 1 µg/ml + Jurkat cell lysate at 50 µg

    Predicted band size : 56 kDa
  • All lanes : Anti-PPP2R5A antibody (ab89621) at 1 µg/ml

    Lane 1 : PPP2R5A transfected 293T cell lysate
    Lane 2 : Untransfected 293T cell lysate

    Lysates/proteins at 25 µg per lane.


    Predicted band size : 56 kDa
    Observed band size : 53 kDa (why is the actual band size different from the predicted?)

プロトコール

参考文献

This product has been referenced in:
  • Greenwood EJ  et al. Temporal proteomic analysis of HIV infection reveals remodelling of the host phosphoproteome by lentiviral Vif variants. Elife 5:N/A (2016). Read more (PubMed: 27690223) »
  • Porter IM  et al. Bod1 regulates protein phosphatase 2A at mitotic kinetochores. Nat Commun 4:2677 (2013). WB ; Human . Read more (PubMed: 24157919) »

See all 2 Publications for this product

レビューと Q&A

Application
Western blot
Loading amount
100000 cells
Gel Running Conditions
Reduced Denaturing (10%)
Sample
Human Cell lysate - whole cell (CCRF-CEM cells)
Specification
CCRF-CEM cells
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
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投稿 Nov 20 2014

Application
Immunocytochemistry/ Immunofluorescence
Blocking step
5% BSA + 1% NDS as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5%
Sample
Human Cell (HeLa)
Specification
HeLa
Permeabilization
Yes - 0.1% Triton X-100
Fixative
Paraformaldehyde
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投稿 Jul 09 2013

Western blot

Excellent
Abreviews
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Loading amount
35 µg
Gel Running Conditions
Reduced Denaturing (4-12% Bis-Tris)
Sample
Human Cell lysate - nuclear (HeLa nuclear cell lysate)
Specification
HeLa nuclear cell lysate
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
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投稿 May 24 2013

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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