製品の概要

  • 製品名Anti-PPP2R5A antibody
    PPP2R5A 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to PPP2R5A
  • アプリケーション適用あり: ICC/IF, WB, IPmore details
  • 種交差性
    交差種: Mouse, Human
    交差が予測される動物種: Rat, Dog, Chimpanzee, Ferret, Rhesus monkey, Gorilla, Orangutan
  • 免疫原

    Synthetic peptide from a region between residue 25 and 75 of human PPP2R5A, NP_006234.1.

  • ポジティブ・コントロール
    • Whole cell lysate from Hela, 293T and NIH 3T3 cells. This antibody gave a positive result when used in the following methanol fixed cell lines: DU145

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab72028 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF
WB
IP
  • 追加情報IP: Use at 2-5µg/mg of lysate.
    WB: 1/2000 - 1/10000. Detects bands of approximately 56 and 250 kDa (predicted molecular weight: 56 kDa).


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • ターゲット情報

    • 機能The B regulatory subunit might modulate substrate selectivity and catalytic activity, and also might direct the localization of the catalytic enzyme to a particular subcellular compartment.
    • 組織特異性Widely expressed with the highest expression in heart and skeletal muscle.
    • 配列類似性Belongs to the phosphatase 2A regulatory subunit B56 family.
    • 翻訳後修飾Phosphorylated on serine residues.
    • 細胞内局在Cytoplasm. Nucleus. Chromosome > centromere. From mitotic prophase to metaphase, localizes at the inner centromere between a pair of sister kinetochores. Decreased expression at the onset of anaphase.
    • Information by UniProt
    • 参照データベース
    • 別名
      • 2A5A_HUMAN antibody
      • B56A antibody
      • MGC131915 antibody
      • PP2A B subunit B' alpha isoform antibody
      • PP2A B subunit B56 alpha isoform antibody
      • PP2A B subunit isoform B''-alpha antibody
      • PP2A B subunit isoform B56-alpha antibody
      • PP2A B subunit isoform PR61-alpha antibody
      • PP2A B subunit isoform R5-alpha antibody
      • PP2A B subunit PR61 alpha isoform antibody
      • PP2A B subunit R5 alpha isoform antibody
      • PPP2R5A antibody
      • PR61A antibody
      • PR61alpha antibody
      • Protein phosphatase 2 regulatory subunit B (B56) alpha isoform antibody
      • Protein phosphatase 2 regulatory subunit B' alpha antibody
      • Serine/threonine-protein phosphatase 2A 56 kDa regulatory subunit alpha isoform antibody
      see all

    Anti-PPP2R5A antibody 画像

    • ICC/IF image of ab72028 stained DU145 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab72028 at 5µg/ml overnight at +4°C. The secondary antibody (pseudo-colored green) was Alexa Fluor® 488 goat anti- rabbit (ab150081) IgG (H+L) preadsorbed, used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (pseudo-colored red) at a 1/200 dilution for 1h at room temperature. DAPI was used to stain the cell nuclei (pseudo-colored blue) at a concentration of 1.43µM for 1hour at room temperature.

    • All lanes : Anti-PPP2R5A antibody (ab72028) at 0.04 µg/ml

      Lane 1 : Whole cell lysate from Hela cells at 50 µg
      Lane 2 : Whole cell lysate from Hela cells at 15 µg
      Lane 3 : Whole cell lysate from Hela cells at 5 µg
      Lane 4 : Whole cell lysate from 293T cells at 50 µg
      Lane 5 : Whole cell lysate from NIH 3T3 cells at 50 µg


      Predicted band size : 56 kDa
      Observed band size : 56 kDa
      Additional bands at : 250 kDa. We are unsure as to the identity of these extra bands.
    • Immunoprecipitation/ Western Blot of PPP2R5A.
      Lane 1: ab72028 at 3µg/mg whole cell lysate.
      Lane 2: Control IgG.
      Whole cell lysate from Hela cells at 1mg for IP, 20% of IP loaded.
      For Western blot detection ab72028 was used at 0.1 µg/ml.
      Chemiluminescence with an exposure time of 30 seconds.
    • IHC image of ab72028 staining in human normal breast formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab72028, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

    Anti-PPP2R5A antibody (ab72028) 使用論文

    This product has been referenced in:
    • DeGrande ST  et al. Molecular mechanisms underlying cardiac protein phosphatase 2A regulation in heart. J Biol Chem 288:1032-46 (2013). WB ; Mouse, Rat, Dog, Human . Read more (PubMed: 23204520) »

    See 1 Publication for this product

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"