製品の概要
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製品名Anti-PPP2R4 antibody
PPP2R4 一次抗体 製品一覧 -
製品の詳細Goat polyclonal to PPP2R4
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由来種Goat
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特異性This antibody is expected to recognize all reported isoforms (NP_821068.1; NP_066954.2; NP_821070.1).
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アプリケーション適用あり: WBmore details
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種交差性交差種: Mouse, Rat, Human
交差が予測される動物種: Rabbit, Cow, Dog, Orangutan
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免疫原
Synthetic peptide:
C-GSLLPIHPVTSG
, corresponding to C terminal amino acids 312-323 of Human PPP2R4 (NP_066954.2). -
ポジティブ･コントロール
- Human, mouse or rat Liver lysate and HepG2 cell lysate.
製品の特性
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製品の状態Liquid
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保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
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バッファーPreservative: 0.02% Sodium Azide
Constituents: 0.5% BSA, Tris saline, pH 7.3 -
Concentration information loading... -
精製度Immunogen affinity purified
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特記事項（精製）ab87673 is purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
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ポリ/モノポリクローナル
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アイソタイプIgG
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研究分野
関連製品
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Compatible Secondaries
アプリケーション
Our Abpromise guarantee covers the use of ab87673 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB | Use a concentration of 0.5 - 2 µg/ml. Detects a band of approximately 37 kDa (predicted molecular weight: 37 kDa). |
ターゲット情報
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機能PPIases accelerate the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides. Acts as a regulatory subunit for serine/threonine-protein phosphatase 2A (PP2A) modulating its activity or substrate specificity, probably by inducing a conformational change in the catalytic subunit, a proposed direct target of the PPIase. Can reactivate inactive phosphatase PP2A-phosphatase methylesterase complexes (PP2A(i)) in presence of ATP and Mg(2+) (By similarity). Reversibly stimulates the variable phosphotyrosyl phosphatase activity of PP2A core heterodimer PP2A(D) in presence of ATP and Mg(2+) (in vitro). The phosphotyrosyl phosphatase activity is dependent of an ATPase activty of the PP2A(D):PPP2R4 complex. Is involved in apoptosis; the function appears to be independent from PP2A.
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組織特異性Widely expressed.
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配列類似性Belongs to the PTPA-type PPIase family.
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細胞内局在Cytoplasm. Nucleus.
- Information by UniProt
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参照データベース
- Entrez Gene: 514460 Cow
- Entrez Gene: 5524 Human
- Entrez Gene: 110854 Mouse
- Entrez Gene: 100009404 Rabbit
- Entrez Gene: 362102 Rat
- Omim: 600756 Human
- SwissProt: Q2KJ44 Cow
- SwissProt: Q15257 Human
see all -
別名
- subunit B'' antibody
- KIAA0044 antibody
- MGC2184 antibody
see all
画像
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Western blot - Anti-PPP2R4 antibody (ab87673)All lanes : Anti-PPP2R4 antibody (ab87673) at 0.5 µg/ml
Lane 1 : Human Liver lysate in RIPA buffer
Lane 2 : Mouse Liver lysate in RIPA buffer
Lane 3 : Rat Liver lysate in RIPA buffer
Lysates/proteins at 35 µg per lane.
Developed using the ECL technique.
Predicted band size: 37 kDa
Observed band size: 37 kDa
プロトコール
参考文献
ab87673 has not yet been referenced specifically in any publications.
レビューと Q&A
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"