製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to PPP2R3B
  • アプリケーション
    適用あり: WB, IPmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Rhesus monkey, Gorilla, Orangutan
  • 免疫原

    Synthetic peptide from a region between residue 350 and 400 of human PPP2R3B, NP_037371.2.

  • ポジティブ・コントロール
    • Whole cell lysate from Hela and 293T cells.

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • バッファー
    Preservative: 0.09% Sodium Azide
    Constituents: 8mM PBS, 60mM Citrate, 150mM Tris, pH 7-8
  • Concentration information loading...
  • 精製度
    Immunogen affinity purified
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab72027 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB
IP
  • 追加情報
    IP: Use at 2-5µg/mg of lysate.
    WB: 1/2000 - 1/10000. Detects bands of approximately 65 and 200 kDa (predicted molecular weight: 65 kDa).


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • ターゲット情報

    • 機能
      The B regulatory subunit might modulate substrate selectivity and catalytic activity, and also might direct the localization of the catalytic enzyme to a particular subcellular compartment.
    • 配列類似性
      Contains 1 EF-hand domain.
    • 細胞内局在
      Nucleus.
    • Information by UniProt
    • 参照データベース
    • 別名
      • NY REN 8 antibody
      • NY REN 8 antigen antibody
      • P2R3B_HUMAN antibody
      • PP2A B'' subunit PR48 antibody
      • PP2A subunit B isoform PR48 antibody
      • PP2A subunit B PR48 isoform antibody
      • PPP2R3B antibody
      • PPP2R3L antibody
      • PPP2R3LY antibody
      • PR 48 antibody
      • PR48 antibody
      • Protein phosphatase 2 (formerly 2A) regulatory subunit B'' beta antibody
      • Protein phosphatase 2 regulatory subunit B'' beta antibody
      • Protein phosphatase 2A 48 kDa regulatory subunit antibody
      • Serine/threonine protein phosphatase 2A 48kDa regulatory subunit B antibody
      • Serine/threonine-protein phosphatase 2A regulatory subunit B'' subunit beta antibody
      see all

    画像

    • All lanes : Anti-PPP2R3B antibody (ab72027) at 0.1 µg/ml

      Lane 1 : Whole cell lysate from Hela cells at 50 µg
      Lane 2 : Whole cell lysate from Hela cells at 15 µg
      Lane 3 : Whole cell lysate from Hela cells at 5 µg
      Lane 4 : Whole cell lysate from 293T cells at 50 µg


      Predicted band size : 65 kDa
      Observed band size : 65 kDa
      Additional bands at : 200 kDa. We are unsure as to the identity of these extra bands.
    • Immunoprecipitation/ Western Blot of PPP2R3B.
      Lane 1: ab72027 at 3µg/mg whole cell lysate.
      Lane 2: Control IgG.
      Whole cell lysate from Hela cells at 1mg for IP, 20% of IP loaded.
      For western blot detection step ab72027 was used at 1 µg/ml.
      Chemiluminescence with an exposure time of 30 seconds.

    参考文献

    ab72027 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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