製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to PPP2R1A
  • アプリケーション
    適用あり: WBmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat, Horse, Guinea pig, Cow, Cat, Dog, Pig, Zebrafish
  • 免疫原

    Synthetic peptide corresponding to a region within the N terminal amino acids 108-157 (AVESLRAISH EHSPSDLEAH FVPLVKRLAG GDWFTSRTSA CGLFSVCYPR) of human PPP2R1A (NP_055040).

  • ポジティブ・コントロール
    • 293T cell lysate

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • バッファー
    Preservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • 精製度
    Immunogen affinity purified
  • 特記事項(精製)
    Purified by peptide affinity chromatography method.
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab86406 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 65 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

ターゲット情報

  • 機能
    The PR65 subunit of protein phosphatase 2A serves as a scaffolding molecule to coordinate the assembly of the catalytic subunit and a variable regulatory B subunit. Required for proper chromosome segregation and for centromeric localization of SGOL1 in mitosis.
  • 配列類似性
    Belongs to the phosphatase 2A regulatory subunit A family.
    Contains 15 HEAT repeats.
  • ドメイン
    Each HEAT repeat appears to consist of two alpha helices joined by a hydrophilic region, the intrarepeat loop. The repeat units may be arranged laterally to form a rod-like structure.
  • 細胞内局在
    Cytoplasm. Chromosome > centromere. Centromeric localization requires the presence of BUB1.
  • Information by UniProt
  • 参照データベース
  • 別名
    • 2AAA_HUMAN antibody
    • 6330556D22Rik antibody
    • Alpha isoform of regulatory subunit A, protein phosphatase antibody
    • DKFZp470L0914 antibody
    • Medium tumor antigen-associated 61 kDa protein antibody
    • MGC128399 antibody
    • MGC786 antibody
    • MGC83000 antibody
    • MGC95083 antibody
    • PP2A Aalpha antibody
    • PP2A antibody
    • PP2A subunit A isoform PR65-alpha antibody
    • PP2A subunit A isoform R1-alpha antibody
    • PP2A, subunit A, PR65-alpha isoform antibody
    • PP2A, subunit A, R1-alpha isoform antibody
    • PP2AAALPHA antibody
    • Ppp2r1a antibody
    • ppp2r1a-b antibody
    • PR65 antibody
    • PR65-alpha antibody
    • PR65A antibody
    • Protein phosphatase 2 (formerly 2A), regulatory subunit A (PR 65), alpha isoform antibody
    • Protein phosphatase 2 (Formerly 2A), regulatory subunit A (PR 65), alpha isoform, isoform CRA_a antibody
    • Protein phosphatase 2 (formerly 2A), regulatory subunit A, antibody
    • Protein phosphatase 2 regulatory subunit A alpha antibody
    • Protein phosphatase 2, 65-KD regulatory subunit A, alpha antibody
    • Protein phosphatase 2, regulatory subunit A, alpha isoform antibody
    • Protein phosphatase 2, structural/regulatory subunit A, alpha; PPP2R1A antibody
    • Serine/threonine-protein phosphatase 2A 65 kDa regulatory subunit A alpha isoform antibody
    see all

画像

  • Anti-PPP2R1A antibody (ab86406) at 1 µg/ml + 293T cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 65 kDa
    Observed band size : 65 kDa
    Additional bands at : 22 kDa,80 kDa. We are unsure as to the identity of these extra bands.

プロトコール

参考文献

ab86406 has not yet been referenced specifically in any publications.

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