Anti-PPP2CB 抗体 (ab72343)
Key features and details
- Rabbit polyclonal to PPP2CB
- Suitable for: WB, IP
- Reacts with: Mouse, Human
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-PPP2CB antibody
PPP2CB 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to PPP2CB -
由来種
Rabbit -
アプリケーション
適用あり: WB, IPmore details -
種交差性
交差種: Mouse, Human
交差が予測される動物種: Rat, Rabbit, Chicken, Guinea pig, Cow, Dog, Turkey, Pig, Xenopus laevis, Chimpanzee, Drosophila melanogaster, Zebrafish, Rhesus monkey, Gorilla, Orangutan, Xenopus tropicalis -
免疫原
Synthetic peptide corresponding to Human PPP2CB. Synthetic peptide from a region between residue 260 and the C-terminus (residue 309) of human PPP2CB
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ポジティブ・コントロール
- Whole cell lysate from Hela, NIH3T3 and 293T cells.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 7
Preservative: 0.09% Sodium azide
Constituents: 1.815% Tris, 1.764% Sodium citrate, 0.021% PBS -
Concentration information loading...
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精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab72343の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
1/2000 - 1/10000. Detects a band of approximately 28, 36, 190 kDa (predicted molecular weight: 36 kDa).
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IP |
Use at 2-5 µg/mg of lysate.
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特記事項 |
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WB
1/2000 - 1/10000. Detects a band of approximately 28, 36, 190 kDa (predicted molecular weight: 36 kDa). |
IP
Use at 2-5 µg/mg of lysate. |
ターゲット情報
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機能
PP2A can modulate the activity of phosphorylase B kinase casein kinase 2, mitogen-stimulated S6 kinase, and MAP-2 kinase. -
配列類似性
Belongs to the PPP phosphatase family. PP-1 subfamily. -
翻訳後修飾
Reversibly methyl esterified on Leu-309. Carboxyl methylation may play a role in holoenzyme assembly. It varies during the cell cycle. Demethylated by PME1 (in vitro).
Phosphorylation of either threonine (by autophosphorylation-activated protein kinase) or tyrosine results in inactivation of the phosphatase. Auto-dephosphorylation has been suggested as a mechanism for reactivation. -
細胞内局在
Cytoplasm. Nucleus. Chromosome > centromere. Cytoplasm > cytoskeleton > spindle pole. In prometaphase cells, but not in anaphase cells, localizes at centromeres. During mitosis, also found at spindle poles. - Information by UniProt
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参照データベース
- Entrez Gene: 524361 Cow
- Entrez Gene: 5516 Human
- Entrez Gene: 19053 Mouse
- Entrez Gene: 397136 Pig
- Entrez Gene: 100009300 Rabbit
- Entrez Gene: 24673 Rat
- Omim: 176916 Human
- SwissProt: Q0P594 Cow
see all -
別名
- PP2A beta antibody
- PP2A-beta antibody
- PP2AB_HUMAN antibody
see all
画像
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All lanes : Anti-PPP2CB antibody (ab72343) at 0.1 µg/ml
Lane 1 : Whole cell lysate from Hela cells at 50 µg
Lane 2 : Whole cell lysate from Hela cells at 15 µg
Lane 3 : Whole cell lysate from Hela cells at 5 µg
Lane 4 : Whole cell lysate from 293T cells at 50 µg
Lane 5 : Whole cell lysate from NIH3T3 cells at 50 µg
Developed using the ECL technique.
Predicted band size: 36 kDa
Observed band size: 36 kDa
Additional bands at: 190 kDa, 28 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 3 minutes -
Immunoprecipitation/ Western Blot of PPP2CB.
Lane 1: ab72343 at 3µg/mg whole cell lysate. Lane 2: Control IgG.
ab72343 at 1µg/ml for WB. Whole cell lysate from Hela cells at 1mg for IP, 20% of IP loaded.
Chemiluminescence with an exposure time of 30 seconds.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (1)
ab72343 は 1 報の論文で使用されています。
- Joseph BK et al. Inhibition of AMP Kinase by the Protein Phosphatase 2A Heterotrimer, PP2APpp2r2d. J Biol Chem 290:10588-98 (2015). PubMed: 25694423