製品の概要

  • 製品名Anti-PPP2CB antibody
    PPP2CB 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to PPP2CB
  • 特異性ab32673 recognises PPP2CB. Does not crossreact with PP1 or other protein phosphatases
  • アプリケーション適用あり: WB, IPmore details
  • 種交差性
    交差種: Mouse, Rat, Rabbit, Human
    交差が予測される動物種: Chicken, Cow, Dog, Xenopus laevis, Caenorhabditis elegans, Drosophila melanogaster, Zebrafish, Cynomolgus Monkey, Dictyostelium discoideum
  • 免疫原

    Synthetic peptide:

    GEPHVTRRTPDYFL

    conjugated to KLH, corresponding to C terminal amino acids 296/309 of Human PPP2CB.

  • ポジティブ・コントロール
    • Rabbit brain membrane cytosol and human A431 RIPA cell lysate.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab32673 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB
IP
  • 追加情報IP: Use at a concentration of 4 - 5 µg/ml.
    Note: Immunoprecipitates PP2A catalytic subunit from rabbit brain membrane extract and and 1mg human A431 RIPA cell lysate.
    Phosphatase assay: Use at an assay dependent dilution.
    WB: Use at a concentration of 0.5 - 2 µg/ml. Predicted molecular weight: 35 kDa.
    Note: Detects PP2A catalytic subunit (~34kD) in rabbit brain membrane cytosol and in human A431 RIPA cell lysate.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • ターゲット情報

    • 機能PP2A can modulate the activity of phosphorylase B kinase casein kinase 2, mitogen-stimulated S6 kinase, and MAP-2 kinase.
    • 配列類似性Belongs to the PPP phosphatase family. PP-1 subfamily.
    • 翻訳後修飾Reversibly methyl esterified on Leu-309. Carboxyl methylation may play a role in holoenzyme assembly. It varies during the cell cycle. Demethylated by PME1 (in vitro).
      Phosphorylation of either threonine (by autophosphorylation-activated protein kinase) or tyrosine results in inactivation of the phosphatase. Auto-dephosphorylation has been suggested as a mechanism for reactivation.
    • 細胞内局在Cytoplasm. Nucleus. Chromosome > centromere. Cytoplasm > cytoskeleton > spindle pole. In prometaphase cells, but not in anaphase cells, localizes at centromeres. During mitosis, also found at spindle poles.
    • Information by UniProt
    • 参照データベース
    • 別名
      • PP2A beta antibody
      • PP2A-beta antibody
      • PP2AB_HUMAN antibody
      • PP2Abeta antibody
      • PP2CB antibody
      • Ppp2cb antibody
      • Protein phosphatase 2 (formerly 2A), catalytic subunit, beta isoform antibody
      • Protein phosphatase 2 catalytic subunit beta isozyme antibody
      • Protein phosphatase 2, catalytic subunit, beta isoform antibody
      • Protein phosphatase 2A catalytic subunit beta isoform antibody
      • Protein phosphatase type 2A catalytic subunit antibody
      • Serine/threonine protein phosphatase 2A catalytic subunit beta antibody
      • Serine/threonine-protein phosphatase 2A catalytic subunit beta isoform antibody
      see all

    Anti-PPP2CB antibody (ab32673) 使用論文

    ab32673 has not yet been referenced specifically in any publications.

    Product Wall

    There are currently no Abreviews or Questions for ab32673.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"