Anti-PPP1A (phospho T320) 抗体 [EP1512Y] (ab62334)

製品の概要

  • 製品名Anti-PPP1A (phospho T320) antibody [EP1512Y]
    PPP1A 一次抗体 製品一覧
  • 製品の詳細
    Rabbit monoclonal [EP1512Y] to PPP1A (phospho T320)
  • 特異性This antibody detects PP1 alpha phosphorylated at Threonine 320.
  • アプリケーション適用あり: WB, IP, IHC-Pmore details
    適用なし: Flow Cyt or ICC
  • 種交差性
    交差種: Mouse, Rat, Human, Xenopus laevis
  • 免疫原

    Phospho specific peptide corresponding to residues surrounding Threonine 320 of human PP1A.

  • ポジティブ・コントロール
    • HeLa cell lysate and human muscle tissue.
  • 特記事項

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab62334 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/1000 - 1/10000. Detects a band of approximately 38 kDa (predicted molecular weight: 38 kDa).
IP 1/40.
IHC-P 1/100 - 1/250. An antigen retrieval step is strongly recommended.
  • 追加情報Is unsuitable for Flow Cyt or ICC.
  • ターゲット情報

    • 機能Protein phosphatase 1 (PP1) is essential for cell division, and participates in the regulation of glycogen metabolism, muscle contractility and protein synthesis. Involved in regulation of ionic conductances and long-term synaptic plasticity. May play an important role in dephosphorylating substrates such as the postsynaptic density-associated Ca(2+)/calmodulin dependent protein kinase II. Component of the PTW/PP1 phosphatase complex, which plays a role in the control of chromatin structure and cell cycle progression during the transition from mitosis into interphase.
    • 配列類似性Belongs to the PPP phosphatase family. PP-1 subfamily.
    • 細胞内局在Cytoplasm. Nucleus. Nucleus > nucleoplasm. Nucleus > nucleolus. Primarily nuclear and largely excluded from the nucleolus. Highly mobile in cells and can be relocalized through interaction with targeting subunits. NOM1 plays a role in targeting this protein to the nucleolus. In the presence of PPP1R8 relocalizes from the nucleus to nuclear speckles.
    • Information by UniProt
    • 参照データベース
    • 別名
      • Alpha isoform serine threonine protein phosphatase PP1alpha 1 catalytic subunit antibody
      • Catalytic subunit antibody
      • EC 3.1.3.16 antibody
      • MGC15877 antibody
      • MGC1674 antibody
      • PP 1A antibody
      • PP-1A antibody
      • PP1A antibody
      • PP1A_HUMAN antibody
      • PP1alpha antibody
      • PP2C ALPHA antibody
      • PP2CA antibody
      • Ppp1ca antibody
      • Protein Phosphatase 2C Alpha Isoform antibody
      • Serine threonine protein phosphatase PP1 alpha catalytic subunit antibody
      • Serine threonine protein phosphatase PP1 alpha catalytic subunit protein phosphatase 1 antibody
      • Serine/threonine-protein phosphatase PP1-alpha catalytic subunit antibody
      see all

    Anti-PPP1A (phospho T320) antibody [EP1512Y] 画像

    • All lanes : Anti-PPP1A (phospho T320) antibody [EP1512Y] (ab62334)

      Lane 1 : HeLa cell lysate (untreated)
      Lane 2 : HeLa cell lysate (treated with Calyculin A)

      Lysates/proteins at 10 µg per lane.

      Secondary
      Goat anti-rabbit HRP conjugate at 1/2000 dilution

      Predicted band size : 38 kDa
      Observed band size : 38 kDa
    • Ab62334 (1/100-1/250) staining human PP1A in human muscle tissue by immunohistochemistry using paraffin embedded tissue.

    Anti-PPP1A (phospho T320) antibody [EP1512Y] (ab62334) 使用論文

    This product has been referenced in:
    • Horiuchi D  et al. MYC pathway activation in triple-negative breast cancer is synthetic lethal with CDK inhibition. J Exp Med 209:679-96 (2012). Read more (PubMed: 22430491) »
    • Cheng G  et al. Basis for MAP4 dephosphorylation-related microtubule network densification in pressure overload cardiac hypertrophy. J Biol Chem 285:38125-40 (2010). WB . Read more (PubMed: 20889984) »

    See all 3 Publications for this product

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    I am very pleased to hear you would like to test ab62334 in rabbit. This code will give you: 1 free primary antibody before the expiration date. To redeem this offer, please submit an Abreview forrabbitand include this code in the “Additional Com...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"