Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human pineal tissue labelling PPME1 with ab86409 at 1/100. A Cy3-conjugated donkey anti-rabbit IgG (1/200) was used as the secondary antibody. Positive staining shown in the cytoplasm of cell bodies of pinealocytes. Magnification: 20X. Exposure time: 0.5 - 2.0 seconds. Left - DAPI. Middle - PPME1. Right - Merge.
Western blot - PPME1 antibody (ab86409)
Anti-PPME1 antibody (ab86409) at 1 µg/ml + 721_B cell lysate at 10 µg
Secondary HRP-conjugated anti-Rabbit IgG at 1/50000 dilution
Predicted band size : 42 kDa Gel concentration 12%