製品の概要

  • 製品名Anti-PPM1A antibody [p6c7]
    PPM1A 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [p6c7] to PPM1A
  • アプリケーション適用あり: IHC-P, ELISA, WBmore details
  • 種交差性
    交差種: Mouse, Human
  • 免疫原

    Recombinant full length protein (Human).

製品の特性

関連製品

アプリケーション

Our Abpromise guarantee covers the use of ab14824 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P
ELISA
WB
  • 追加情報ELISA: Use at an assay dependent dilution.
    WB: 1/250 - 1/1000. Predicted molecular weight: 42 kDa.
    IHC-P: Use at a concentration of 4 µg/ml.

    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • ターゲット情報

    • 機能Enzyme with a broad specificity. Negatively regulates TGF-beta signaling through dephosphorylating SMAD2 and SMAD3, resulting in their dissociation from SMAD4, nuclear export of the SMADs and termination of the TGF-beta-mediated signaling.
    • 配列類似性Belongs to the PP2C family.
    • 細胞内局在Nucleus.
    • Information by UniProt
    • 参照データベース
    • 別名
      • Protein phosphatase 2C isoform alpha antibody
      • EC 3.1.3.16 antibody
      • FLJ42306 antibody
      • IA antibody
      • MGC9201 antibody
      • Mpp alpha antibody
      • PP2C alpha antibody
      • PP2C-alpha antibody
      • PP2CA antibody
      • PP2Calpha antibody
      • PPM 1A antibody
      • PPM1A antibody
      • PPM1A_HUMAN antibody
      • PPPM1A antibody
      • Protein phosphatase 1A (formerly 2C) magnesium dependent alpha isoform antibody
      • Protein phosphatase 1A antibody
      • Protein phosphatase 1A magnesium dependent alpha antibody
      • Protein phosphatase 2C alpha antibody
      • Protein phosphatase 2C alpha isoform antibody
      • Protein phosphatase 2C isoform alpha antibody
      • Protein phosphatase IA antibody
      • Protein phosphatase, Mg2+/Mn2+ dependent, 1A antibody
      see all

    Anti-PPM1A antibody [p6c7] 画像



    • Predicted band size : 42 kDa

      Lane 1: Wild-type HAP1 cell lysate (20 µg)
      Lane 2: PPM1A knockout HAP1 cell lysate (20 µg)
      Lane 3: HeLa cell lysate (20 µg)
      Lane 4: Jurkat cell lysate (20 µg)
      Lanes 1 - 4: Merged signal (red and green). Green - ab14824 observed at 42 kDa. Red - loading control, ab18251, observed at 52 kDa.

      ab14824 was shown to specifically react with PPM1A when PPM1A knockout samples were used. Wild-type and PPM1A knockout samples were subjected to SDS-PAGE. ab14824 diluted to 1/250 and ab18251 (loading control to alpha Tubulin) diluted to 1/10,000 were incubated overnight at 4°C. Blots were developed with goat anti-rabbit IgG (H + L) and goat anti-mouse IgG (H + L) secondary antibodies at 1/10,000 dilution for 1 hour at room temperature before imaging.



    • Predicted band size : 42 kDa
      Western blot analysis of mouse liver cytosol extract using ab14824 at a dilution of 1/250.  Proteins were visualised using a goat anti-mouse secondary antibody conjugated to HRP and a DAB detection system. Western blot analysis of mouse liver cytosol extract using ab14824 at a dilution of 1/250. Proteins were visualised using a goat anti-mouse secondary antibody conjugated to HRP and a DAB detection system.
    • Anti-PPM1A antibody [p6c7] (ab14824) at 1/1000 dilution + HeLa whole cell lysate

      Secondary
      HRP conjugated anti-mouse antibody
      Developed using the ECL technique

      Performed under reducing conditions.

      Predicted band size : 42 kDa
      Observed band size : 45 kDa (why is the actual band size different from the predicted?)


      Exposure time : 45 s
      This image is courtesy of an Abreview submitted by Xia Lin on 2 March 2006.

      See Abreview

    • Ab14824 staining human colon. Staining is localised to cytoplasm.
      Left panel: with primary antibody at 4ug/ml. Right panel: isotype control.
      Sections were stained using an automated system DAKO Autostainer Plus , at room temperature. Sections were rehydrated and antigen retrieved with the Dako 3-in-1 antigen retrieval buffer EDTA pH 9.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 minutes. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 minutes and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.

    Anti-PPM1A antibody [p6c7] (ab14824) 使用論文

    This product has been referenced in:
    • Kim YG  et al. Role of protein phosphatase magnesium-dependent 1A and anti-protein phosphatase magnesium-dependent 1A autoantibodies in ankylosing spondylitis. Arthritis Rheumatol 66:2793-803 (2014). Read more (PubMed: 24980965) »
    • Budhiraja S  et al. Phosphatase PPM1A negatively regulates P-TEFb function in resting CD4T+ T cells and inhibits HIV-1 gene expression. Retrovirology 9:52 (2012). Read more (PubMed: 22727189) »

    See all 9 Publications for this product

    Product Wall

    Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products. I am sorry this product did not perform as stated on the datasheet and for the inconvenience this h...

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    Thank you for contacting Abcam regarding ab14824. I am sorry that you are experiencing difficulties with the most recent lot that you received. As this is a monoclonal antibody, I would not expect much lot to lot variation, but I would advise two m...

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    Thank you for your enquiry. I am sorry we regrettably haven’t tested the cross reactivity of ab14824 PPM1A antibody [p6c7] with other PP2C isoforms. By looking at the Western Blot image on the datasheet, we believe there’s a possibility that it is ...

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    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Western blot
    Sample Human Cell lysate - other (aortic smooth muscle cells (cytoplasmic))
    Loading amount 10 µg
    Specification aortic smooth muscle cells (cytoplasmic)
    Gel Running Conditions Reduced Denaturing (Bis-Tris 4-12% MOPS running buffer)
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
    Username

    Abcam user community

    Verified customer

    投稿 Apr 09 2008

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Western blot
    Sample Human Cell lysate - whole cell (HeLa)
    Specification HeLa
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5%
    Username

    Xia Lin

    Verified customer

    投稿 Mar 02 2006

    Thank you for your enquiry. Unfortunately this antibody has not been tested for cross-reactivity with other isoforms. If you have any additional questions, please contact us again.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"