製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to PPHLN1
  • 特異性
    ab69569 detects endogenous levels of total PPHLN protein.
  • アプリケーション
    適用あり: ICC/IF, WB, ELISA, IHC-Pmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse
  • 免疫原

    Synthesized peptide derived from an internal region of human PPHLN1.

  • ポジティブ・コントロール
    • Extracts from COLO or 293 cells. Liver carcinoma tissue.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab69569 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF Use at an assay dependent concentration.
WB 1/500 - 1/1000. Detects a band of approximately 53 kDa (predicted molecular weight: 53 kDa).
ELISA 1/20000.
IHC-P 1/50 - 1/100.

ターゲット情報

  • 機能
    Involved in epithelial differentiation and contributes to epidermal integrity and barrier formation.
  • 組織特異性
    Ubiquitous.
  • 翻訳後修飾
    Substrate of transglutaminase (in vitro).
  • 細胞内局在
    Nucleus. Cytoplasm. In undifferentiated keratinocytes expressed in speckle-type nuclear granules and at the nuclear membrane, but in the differentiated keratinocytes colocalized with periplakin at the cell periphery and at cell-cell junctions.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Gastric cancer antigen Ga50 antibody
    • Periphilin 1 antibody
    • Periphilin-1 antibody
    • PPHLN_HUMAN antibody
    • PPHLN1 antibody
    see all

画像

  • All lanes : Anti-PPHLN1 antibody (ab69569) at 1/500 dilution

    Lane 1 : Extracts from COLO cells
    Lane 2 : Extracts from 293 cells
    Lane 3 : Extracts from COLO cells with immunizing peptide at 10 µg

    Lysates/proteins at 30 µg per lane.


    Predicted band size : 53 kDa
    Observed band size : 53 kDa
    Additional bands at : 100 kDa,35 kDa,40 kDa,55 kDa,85 kDa. We are unsure as to the identity of these extra bands.
  • Immunohistochemistry analysis of paraffin-embedded human liver carcinoma tissue using 1/50-1/100 ab69569. For the image in the right panel, the antibody was preincubated with the immunizing peptide.

参考文献

This product has been referenced in:

See 1 Publication for this product

レビューと Q&A

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (HeLa)
Specification
HeLa
Fixative
Formaldehyde
Permeabilization
Yes - 0.5% Triton X-100
Blocking step
BSA as blocking agent for 15 minute(s) · Concentration: 5% · Temperature: 20°C
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Verified customer

投稿 Nov 01 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (HeLa)
Loading amount
40 µg
Specification
HeLa
Gel Running Conditions
Reduced Denaturing (8%)
Blocking step
BSA as blocking agent for 45 minute(s) · Concentration: 5% · Temperature: 20°C
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Abcam user community

Verified customer

投稿 Oct 31 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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