Anti-PPAR gamma 1+2 抗体 [A3409A] - ChIP Grade (ab41928)

製品の概要

  • 製品名Anti-PPAR gamma 1+2 antibody [A3409A] - ChIP Grade
  • 製品の詳細
    Mouse monoclonal [A3409A] to PPAR gamma 1+2 - ChIP Grade
  • 特異性This antibody specifically recognizes PPAR gamma 1 and 2. The specificity for each subtype regarding to alpha, gamma -1, -2 , delta was confirmed by each cDNA transfected to CHO cell and the lysate reacted with corresponding antibody in a panel; the antibody was found not to react with alpha or delta forms.
  • アプリケーション適用あり: IHC-P, WB, ELISA, IP, ChIPmore details
  • 種交差性
    交差種: Mouse, Rat, Human
  • 免疫原

    Baculovirus-expressed recombinant fragment, corresponding to amino acids 3-105 of Human PPAR gamma 1+2.

  • ポジティブ・コントロール
    • IHC: Rat adipose cells and rat placenta. WB: Nuclear lysate of 3T3l-1 cells induced for adipocyte differentiation

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab41928 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P Use a concentration of 10 µg/ml. Perform antigen retrieval by autoclave to preserve reproducibility.
Sections should be fixed with formalin for no longer than 48 hours.
EMSA Use at an assay dependent concentration.
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 58 kDa. A nuclear extraction is recommended.
ELISA Use a concentration of 0.012 µg/ml.
IP Use at an assay dependent concentration.
ChIP Use at an assay dependent concentration. PubMed: 16197558

ターゲット情報

  • 機能Receptor that binds peroxisome proliferators such as hypolipidemic drugs and fatty acids. Once activated by a ligand, the receptor binds to a promoter element in the gene for acyl-CoA oxidase and activates its transcription. It therefore controls the peroxisomal beta-oxidation pathway of fatty acids. Key regulator of adipocyte differentiation and glucose homeostasis.
  • 組織特異性Highest expression in adipose tissue. Lower in skeletal muscle, spleen, heart and liver. Also detectable in placenta, lung and ovary.
  • 関連疾患Note=Defects in PPARG can lead to type 2 insulin-resistant diabetes and hyptertension. PPARG mutations may be associated with colon cancer.
    Defects in PPARG may be associated with susceptibility to obesity (OBESITY) [MIM:601665]. It is a condition characterized by an increase of body weight beyond the limitation of skeletal and physical requirements, as the result of excessive accumulation of body fat.
    Defects in PPARG are the cause of familial partial lipodystrophy type 3 (FPLD3) [MIM:604367]. Familial partial lipodystrophies (FPLD) are a heterogeneous group of genetic disorders characterized by marked loss of subcutaneous (sc) fat from the extremities. Affected individuals show an increased preponderance of insulin resistance, diabetes mellitus and dyslipidemia.
    Genetic variations in PPARG can be associated with susceptibility to glioma type 1 (GLM1) [MIM:137800]. Gliomas are central nervous system neoplasms derived from glial cells and comprise astrocytomas, glioblastoma multiforme, oligodendrogliomas, and ependymomas. Note=Polymorphic PPARG alleles have been found to be significantly over-represented among a cohort of American patients with sporadic glioblastoma multiforme suggesting a possible contribution to disease susceptibility.
  • 配列類似性Belongs to the nuclear hormone receptor family. NR1 subfamily.
    Contains 1 nuclear receptor DNA-binding domain.
  • 細胞内局在Nucleus.
  • Information by UniProt
  • 参照データベース
  • 別名
    • NR1C3 antibody
    • Nuclear receptor subfamily 1 group C member 3 antibody
    • Peroxisome proliferator-activated receptor gamma antibody
    • PPAR gamma antibody
    • PPAR-gamma antibody
    • PPARG antibody
    • PPARG_HUMAN antibody
    • PPARG1 antibody
    • PPARG2 antibody
    see all

Anti-PPAR gamma 1+2 antibody [A3409A] - ChIP Grade 画像

  • ab41928 staining PPAR gamma 1+2 in rat adiposoe cells (10 ug/mL) by immunohistochemistry, formalin-fixed paraffin embedded sections.
  • All lanes : Anti-PPAR gamma 1+2 antibody [A3409A] - ChIP Grade (ab41928) at 1 µg/ml

    Lane 1 : Nuclear lysate of non induced 3T3l-1 cells
    Lane 2 : Nuclear lysate of 3T3l-1 cells induced for adipocyte differentiation

    Lysates/proteins at 20 µg per lane.


    Predicted band size : 58 kDa
    Two bands are visible: PPAR gamma 1 and PPAR gamma 2.
  • Lane 1: FLAG-M2
    Lane 2: ab41928
    Lane 3: ab41928
    Lane 4: Control IgG

    Immunoprecipitation analysis using Fluorescence labeled PPAR gamma generated in vitro translation. Anti-PPAR gamma antibody (ab41928) is diluted with translation production and mixed with protein G Sepharose beads. It is then rotated at 4oC for 1 hour and precipitates are eluted with addition of SDS loading buffer. Samples are incubated for 10minutes at 70°C and analyzed with Fluorescence imager.

  • ab41928 staining PPAR gamma 1+2 in rat plancenta(10 ug/mL) by immunohistochemistry, formalin-fixed paraffin embedded sections.

Anti-PPAR gamma 1+2 antibody [A3409A] - ChIP Grade (ab41928) 使用論文

This product has been referenced in:
  • Chen J  et al. The role and possible mechanism of lncRNA U90926 in modulating 3T3-L1 preadipocyte differentiation. Int J Obes (Lond) 41:299-308 (2017). WB . Read more (PubMed: 27780975) »
  • Liu Z  et al. Mark4 promotes oxidative stress and inflammation via binding to PPAR? and activating NF-?B pathway in mice adipocytes. Sci Rep 6:21382 (2016). ChIP ; Mouse . Read more (PubMed: 26888669) »

See all 8 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Rat Tissue lysate - whole (fat)
Gel Running Conditions Non-reduced Non-Denaturing (Native) (gel 10%)
Loading amount 20 µg
Specification fat
Blocking step BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 26°C
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投稿 Mar 14 2017

Application Western blot
Sample Mouse Tissue lysate - whole (liver)
Gel Running Conditions Non-reduced Non-Denaturing (Native) (10%gel)
Loading amount 15 µg
Specification liver
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 20% · Temperature: 25°C
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投稿 Aug 19 2015

Proteins that are found exclusively or predominantly in a sub-cellular location can be enriched in a lysate of the sub-cellular fraction compared to whole cell or tissue lysates. This can be useful when trying to obtain a signal for a weakly-expressed ...

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I am very pleased to hear you would like to accept our offer and test ab41928 in Flow Cytometry. This code will give you xxxxxxxxx off your next order before the expi...

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Thank you for contacting us.
Regarding PPAR-delta ab:
I think ab58137 (www.abcam.com/ab58137) as you suggested would be the best antibody to try as it has been tested in flow cyt and using the same secondary ab (ab96879) would be fine. Howeve...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"