製品の概要

  • 製品名Anti-PPAR alpha antibody
    PPAR alpha 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to PPAR alpha
  • 特異性While the localization of the protein seems to be widely regarded as nuclear, there are several publications documenting cytoplasmic localization of this protein in a number of other cell types: e.g., pubmed ID 16875506 (lymphocytes), 16875506 (NIH3T3), 9748221 (macrophages), and 10766862 (chondrocytes).
  • アプリケーション適用あり: ELISA, ICC/IF, IHC-P, WB, IHC-Fr, IHC-FoFrmore details
  • 種交差性
    交差種: Mouse, Rat, Human
    交差が予測される動物種: Cow, Dog
  • 免疫原

    Synthetic peptide corresponding to Mouse PPAR alpha aa 1-18 (N terminal).
    Sequence:

    MVDTESPICPLSPLEADD


    Database link: P23204

  • ポジティブ・コントロール
    • Mouse 3T3 cells Rat brain tissue

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab8934 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ELISA 1/8000 - 1/32000.
ICC/IF Use a concentration of 1 µg/ml.
IHC-P 1/200. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
WB 1/500 - 1/2000. Detects a band of approximately 52 kDa (predicted molecular weight: 52 kDa). We recommend overnight blocking with BSA solution at 4C, and incubating with the primary antibody overnight.
IHC-Fr 1/1000. PubMed: 17405874
IHC-FoFr Use at an assay dependent concentration.

ターゲット情報

  • 機能Ligand-activated transcription factor. Key regulator of lipid metabolism. Activated by the endogenous ligand 1-palmitoyl-2-oleoyl-sn-glycerol-3-phosphocholine (16:0/18:1-GPC). Activated by oleylethanolamide, a naturally occurring lipid that regulates satiety (By similarity). Receptor for peroxisome proliferators such as hypolipidemic drugs and fatty acids. Regulates the peroxisomal beta-oxidation pathway of fatty acids. Functions as transcription activator for the ACOX1 and P450 genes. Transactivation activity requires heterodimerization with RXRA and is antagonized by NR2C2.
  • 組織特異性Skeletal muscle, liver, heart and kidney.
  • 配列類似性Belongs to the nuclear hormone receptor family. NR1 subfamily.
    Contains 1 nuclear receptor DNA-binding domain.
  • 細胞内局在Nucleus.
  • Information by UniProt
  • 参照データベース
  • 別名
    • hPPAR antibody
    • MGC2237 antibody
    • MGC2452 antibody
    • NR1C1 antibody
    • Nuclear receptor subfamily 1 group C member 1 antibody
    • OTTHUMP00000197740 antibody
    • OTTHUMP00000197741 antibody
    • Peroxisome proliferative activated receptor alpha antibody
    • Peroxisome proliferator activated receptor alpha antibody
    • Peroxisome proliferator-activated receptor alpha antibody
    • PPAR antibody
    • PPAR-alpha antibody
    • ppara antibody
    • PPARA_HUMAN antibody
    • PPARalpha antibody
    see all

Anti-PPAR alpha antibody 画像



  • Predicted band size : 52 kDa

    Western Blot using ab8934 on 20 ug / lane 3T3 Whole Cell Lysate (ab7179). Goat anti-rabbit IgG HRP (ab6721) Conjugate used as secondary at 1/2000. Exposure time: 10 mins.

    Lane 1: 1/500

    Lane 2: 1/1000

    Western Blot using ab8934 on 20 ug / lane 3T3 Whole Cell Lysate (ab7179). Goat anti-rabbit IgG HRP (ab6721) Conjugate used as secondary at 1/2000. Exposure time: 10 mins. Lane 1: 1/500 Lane 2: 1/1000

  • ab8934 staining PPARα in serum starved HepG2 cells (ab7900) treated with telmisartan (ab120831), by ICC/IF. Increase in PPARα expression correlates with increased concentration of telmisartan, as described in literature.
    The cells were incubated at 37°C for 6h in media containing different concentrations of ab120831 (telmisartan) in DMSO, fixed with 100% methanol for 5 minutes at -20°C and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab8934 (5 µg/ml) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 goat anti-rabbit polyclonal antibody (ab96899) at 1/250 dilution was used as the secondary antibody. Nuclei were counterstained with DAPI and are shown in blue.

  • ab8934 staining of PPAR alpha in rat brain (ab29475) sections, highlighting cytoplasmic staining in ependymal cells and neurons in frontal cortex. Top image shows subventricular zone (svz) of lateral ventrical (exit point of progenitor olfactory neurones); lower image shows frontal cortex in the same section. Cytoplasmic staining is also observed in the corpus callosum (top image) and in dendritic fields of the cortex. Formalin/PFA-fixed paraffin-embedded sections of rat brain tissue (ab29475) were incubated with ab8934 (1/200) for 1 hour. Antigen retrieval was performed by heat induction in citrate buffer pH 6.0. Please see accompanying abreview for additional information.

  • ICC/IF image of ab8934 stained HepG2 cells (ab7900). The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum (ab7481) / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab8934, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • ab8934 staining PPAR in Rat brain tissue (ab29475) sections by Immunohistochemistry (PFA perfusion fixed frozen sections). Tissue samples were fixed by perfusion with acetone, permeablized with methanol and blocked with 5% BSA for 1 hour at 37°C. The sample was incubated with primary antibody (1/100 in PBS) at 4°C for 18 hours. An Alexa Fluor® 488-conjugated Goat anti-rabbit polyclonal (1/200) (ab150077) was used as the secondary antibody.

    See Abreview

  • ab8934 staining PPAR alpha in mouse liver tissue section by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). Tissue underwent formaldehyde fixation before enzymatic antigen retrieval with Protease 0.05% in PBS for 5 min and then blocking with 5% serum was performed for 20 minutes at 20°C. The primary antibody was diluted 1/50 and incubated with sample in Tris + 5% normal goat serum for 1 hour at 20°C. A Biotin conjugated goat polyclonal to rabbit IgG was used at dilution at 1/500 as secondary antibody. Images show nuclear staining in hepatocytes (perfusion-fixed mouse, 10 and 40x microscope magnification).

    See Abreview

Anti-PPAR alpha antibody (ab8934) 使用論文

This product has been referenced in:
  • Randriamboavonjy JI  et al. Cardiac Protective Effects of Moringa oleifera Seeds in Spontaneous Hypertensive Rats. Am J Hypertens 29:873-81 (2016). Read more (PubMed: 26864583) »
  • Li XH  et al. Neuroprotective effects of sodium hydrosulfide against ß-amyloid-induced neurotoxicity. Int J Mol Med 38:1152-60 (2016). WB ; Rat . Read more (PubMed: 27511125) »

See all 23 Publications for this product

Product Wall

Application Western blot
Sample Rat Tissue lysate - whole (LIVER)
Gel Running Conditions Reduced Denaturing
Loading amount 50 µg
Specification LIVER
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

投稿 Oct 11 2016

Application Western blot
Loading amount 30 µg
Gel Running Conditions Reduced Denaturing
Sample Mouse Tissue lysate - nuclear (Mouse Hippocampus)
Specification Mouse Hippocampus
Blocking step Li-Cor Block Buffer as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 21°C
Username

Dr. Grant Corbett

Verified customer

投稿 Jul 25 2014

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Loading amount 100 µg
Gel Running Conditions Reduced Denaturing (10% Gel)
Sample Rat Tissue lysate - whole (brain tissue)
Specification brain tissue
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 37°C
Username

Abcam user community

Verified customer

投稿 Sep 17 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (PFA perfusion fixed frozen sections)
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 37°C
Sample Rat Tissue sections (Brain sections)
Specification Brain sections
Permeabilization Yes - Methanol
Fixative Acetone
Username

Abcam user community

Verified customer

投稿 Sep 16 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 37°C
Sample Rat Cell (Astrocytes)
Specification Astrocytes
Permeabilization Yes - Methanol
Fixative Paraformaldehyde
Username

Abcam user community

Verified customer

投稿 Sep 16 2013

Thank you for contacting us.

We do not have specific protocol for blood related samples where use of ab97609 and ab8934 is documented. The choice of protocol depends on the researcher how they would like to use these antibodies e.g. if you are...

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He llevado a cabo una búsqueda de anticuerpos que pudieran serviros para detectar las proteínas indicadas por WB en lisados de células procedentes de conejo.

Contra PPAR alpha ...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"