Anti-PPAR alpha 抗体 (ab61182)

製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to PPAR alpha
  • 由来種
    Rabbit
  • 特異性
    ab61182 detects PPAR alpha protein, (SwissProt Q07869).
  • アプリケーション
    適用あり: ELISA, ICC/IF, WBmore details
  • 種交差性
    交差種: Mouse, Hamster, Human
  • 免疫原

    Synthetic non-phosphopeptide derived from human PPAR alpha around the phosphorylation site of serine 21 (L-E-SP-P-L).

  • ポジティブ・コントロール
    • NIH 3T3 cell extracts and HeLa cells.

アプリケーション

Our Abpromise guarantee covers the use of ab61182 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ELISA 1/5000.
ICC/IF 1/100 - 1/500.
WB 1/500 - 1/1000. Detects a band of approximately 52 kDa (predicted molecular weight: 52 kDa).

ターゲット情報

  • 機能
    Ligand-activated transcription factor. Key regulator of lipid metabolism. Activated by the endogenous ligand 1-palmitoyl-2-oleoyl-sn-glycerol-3-phosphocholine (16:0/18:1-GPC). Activated by oleylethanolamide, a naturally occurring lipid that regulates satiety (By similarity). Receptor for peroxisome proliferators such as hypolipidemic drugs and fatty acids. Regulates the peroxisomal beta-oxidation pathway of fatty acids. Functions as transcription activator for the ACOX1 and P450 genes. Transactivation activity requires heterodimerization with RXRA and is antagonized by NR2C2.
  • 組織特異性
    Skeletal muscle, liver, heart and kidney.
  • 配列類似性
    Belongs to the nuclear hormone receptor family. NR1 subfamily.
    Contains 1 nuclear receptor DNA-binding domain.
  • 細胞内局在
    Nucleus.
  • Information by UniProt
  • 参照データベース
  • 別名
    • hPPAR antibody
    • MGC2237 antibody
    • MGC2452 antibody
    • NR1C1 antibody
    • Nuclear receptor subfamily 1 group C member 1 antibody
    • OTTHUMP00000197740 antibody
    • OTTHUMP00000197741 antibody
    • Peroxisome proliferative activated receptor alpha antibody
    • Peroxisome proliferator activated receptor alpha antibody
    • Peroxisome proliferator-activated receptor alpha antibody
    • PPAR antibody
    • PPAR-alpha antibody
    • ppara antibody
    • PPARA_HUMAN antibody
    • PPARalpha antibody
    see all

画像

  • ab61182 at 1/100 - 1/500 dilution staining PPAR alpha in HeLa cells by Immunofluorescence, in the absence or presence of the immunising peptide.
  • All lanes : Anti-PPAR alpha antibody (ab61182) at 1/500 dilution

    Lane 1 : NIH 3T3 cell extracts
    Lane 2 : NIH 3T3 cell extracts with the immunising peptide

    Predicted band size: 52 kDa
    Observed band size: 52 kDa

参考文献

ab61182 has not yet been referenced specifically in any publications.

レビューと Q&A

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Application
Immunocytochemistry/ Immunofluorescence
Sample
Hamster Cell (Expression of Human PPAR Alpha in CHO)
Specification
Expression of Human PPAR Alpha in CHO
Fixative
Formaldehyde
Permeabilization
Yes - 0.1 % Triton X-100
Blocking step
TBS + 3% Dried Milk + 0.1 % Triton X-100 as blocking agent for 20 minute(s) · Concentration: 3%
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投稿 Jul 29 2008

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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