製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to PPAR alpha
  • 特異性
    The antibody recognizes ~52 kDa PPARa in human sample. A ~28 kDa cleavage fragment can also be detected in samples of human, mouse, and rat origins.
  • アプリケーション
    適用あり: IP, WBmore details
  • 種交差性
    交差種: Mouse, Rat, Human
    交差が予測される動物種: Dog
  • 免疫原

    Synthetic peptide surrounding amino acid 25 of human PPARa.

  • ポジティブ・コントロール
    • Rat liver/ kidney Baboon myometrium single band at ~52 kDa. K562 cells three bands: 52 kDa band and ~60 kDa band have the similar density and a much weaker band at ~45-50 kDa.

法規制情報

アプリケーション

Our Abpromise guarantee covers the use of ab24509 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IP Use at 12.5-1000 µg/mg of lysate.
WB Use a concentration of 3 - 6 µg/ml. Detects a band of approximately 52 kDa (predicted molecular weight: 52 kDa).

ターゲット情報

  • 機能
    Ligand-activated transcription factor. Key regulator of lipid metabolism. Activated by the endogenous ligand 1-palmitoyl-2-oleoyl-sn-glycerol-3-phosphocholine (16:0/18:1-GPC). Activated by oleylethanolamide, a naturally occurring lipid that regulates satiety (By similarity). Receptor for peroxisome proliferators such as hypolipidemic drugs and fatty acids. Regulates the peroxisomal beta-oxidation pathway of fatty acids. Functions as transcription activator for the ACOX1 and P450 genes. Transactivation activity requires heterodimerization with RXRA and is antagonized by NR2C2.
  • 組織特異性
    Skeletal muscle, liver, heart and kidney.
  • 配列類似性
    Belongs to the nuclear hormone receptor family. NR1 subfamily.
    Contains 1 nuclear receptor DNA-binding domain.
  • 細胞内局在
    Nucleus.
  • Information by UniProt
  • 参照データベース
  • 別名
    • hPPAR antibody
    • MGC2237 antibody
    • MGC2452 antibody
    • NR1C1 antibody
    • Nuclear receptor subfamily 1 group C member 1 antibody
    • OTTHUMP00000197740 antibody
    • OTTHUMP00000197741 antibody
    • Peroxisome proliferative activated receptor alpha antibody
    • Peroxisome proliferator activated receptor alpha antibody
    • Peroxisome proliferator-activated receptor alpha antibody
    • PPAR antibody
    • PPAR-alpha antibody
    • ppara antibody
    • PPARA_HUMAN antibody
    • PPARalpha antibody
    see all

画像

  • All lanes : Anti-PPAR alpha antibody (ab24509) at 0.5 µg/ml

    Lane 1 : Jurkat Cells
    Lane 2 : Jurkat Cells
    Lane 3 : Rat Liver
    Lane 4 : Rat Kidney


    Predicted band size : 52 kDa

参考文献

This product has been referenced in:
  • Mukherjee S  et al. Boosting efferocytosis in alveolar space using BCG vaccine to protect host against influenza pneumonia. PLoS One 12:e0180143 (2017). WB ; Mouse . Read more (PubMed: 28686604) »
  • Fu Y  et al. Tribbles homolog 1 enhances cholesterol efflux from oxidized low-density lipoprotein-loaded THP-1 macrophages. Exp Ther Med 14:862-866 (2017). Read more (PubMed: 28673011) »

See all 15 Publications for this product

レビューと Q&A

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Mouse Tissue lysate - whole (mouse heart)
Loading amount
50 µg
Specification
mouse heart
Gel Running Conditions
Reduced Denaturing
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: rt°C
Username

Abcam user community

Verified customer

投稿 Jun 23 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Mouse Cell lysate - whole cell (C2C12)
Loading amount
40 µg
Specification
C2C12
Gel Running Conditions
Reduced Denaturing
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

投稿 Nov 07 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunoprecipitation
Sample
Mouse Cell lysate - whole cell (Hepa1-6 (murine hepatoma) cells)
Total protein in input
1000 µg
Specification
Hepa1-6 (murine hepatoma) cells
Immuno-precipitation step
Protein A
Username

Abcam user community

Verified customer

投稿 Apr 12 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Mouse Cell lysate - whole cell (Hepa1-6 (murine hepatoma) cells)
Loading amount
50 µg
Specification
Hepa1-6 (murine hepatoma) cells
Gel Running Conditions
Reduced Denaturing
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: 25°C
Username

Abcam user community

Verified customer

投稿 Apr 12 2011

Thank you for your enquiry. After contacting the originator of this antibody, I have confirmed that this antibody does in fact react with rat, and that change will be made to the datasheet. I would expect you to get the predicted band if you used rat s...

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Thank you for getting back to me. From literature searching I can see that the detection of PPAR alpha in rat muscle is to be expected. Given that your customer has been preparing their samples in the same manner and has been detecting the non spec...

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Thank you for your enquiry. I am sorry to hear that you have been having difficulties with this antibody. I have read your technical questionaire and I have a few comments. On the antibody datasheet it details that when using a human erythroid cell ...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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