製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to PPAP2A
  • 特異性
    The immunogen for ab58745 is derived from a region specifically from isoform 2. This sequence shows low homology with isoform 1.
  • アプリケーション
    適用あり: WB, IHC-P, ELISAmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Rat, Guinea pig, Cat
  • 免疫原

    Synthetic peptide within Human PPAP2A aa 31-80 (N terminal). The exact sequence is proprietary. Isoform 2
    Sequence:

    QIYPFQRGFFCKDNSINYPYHDSTVTSTVLILVGVGLPISSIILGETLSV

  • ポジティブ・コントロール
    • HepG2 cell lysate or human kidney tissue.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab58745 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 5 µg/ml. Detects a band of approximately 36 kDa (predicted molecular weight: 32 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
IHC-P Use a concentration of 4 - 8 µg/ml.
ELISA Use at an assay dependent concentration.

ターゲット情報

  • 機能
    Broad-specificity phosphohydrolase that dephosphorylates exogenous bioactive glycerolipids and sphingolipids. Catalyzes the conversion of phosphatidic acid (PA) to diacylglycerol (DG). Pivotal regulator of lysophosphatidic acid (LPA) signaling in the cardiovascular system. Major enzyme responsible of dephosphorylating LPA in platelets, which terminates signaling actions of LPA. May control circulating, and possibly also regulate localized, LPA levels resulting from platelet activation. It has little activity towards ceramide-1-phosphate (C-1-P) and sphingosine-1-phosphate (S-1-P). The relative catalytic efficiency is LPA > PA > S-1-P > C-1-P. It's down-regulation may contribute to the development of colon adenocarcinoma.
  • 組織特異性
    Ubiquitously expressed with highest expression found in prostate. Isoform 1 is predominant in kidney, lung, placenta and liver. Isoform 2 is predominant in heart and pancreas. Found to be down-regulated in colon adenocarcinomas.
  • 配列類似性
    Belongs to the PA-phosphatase related phosphoesterase family.
  • 翻訳後修飾
    N-glycosylated. Contains high-mannose oligosaccharides.
  • 細胞内局在
    Cell membrane.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Lipid phosphate phosphohydrolase 1 antibody
    • Lipid phosphate phosphohydrolase 1a antibody
    • LLP1a antibody
    • LPP1 antibody
    • LPP1_HUMAN antibody
    • PAP 2a antibody
    • PAP-2a antibody
    • PAP2 antibody
    • PAP2-alpha antibody
    • PAP2a antibody
    • PAP2a2 antibody
    • PAP2alpha2 antibody
    • PAPalpha1 antibody
    • Phosphatidate phosphohydrolase type 2a antibody
    • Phosphatidic acid phosphatase 2a antibody
    • Phosphatidic acid phosphatase type 2A antibody
    • Phosphatidic acid phosphohydrolase type 2a antibody
    • PPAP2A antibody
    • Type 2 phosphatidic acid phosphatase alpha antibody
    • Type 2 phosphatidic acid phosphohydrolase antibody
    see all

画像

  • Anti-PPAP2A antibody (ab58745) at 5 µg/ml + HepG2 cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/ 50,000 - 1/100,000 dilution.

    Predicted band size : 32 kDa
    Observed band size : 36 kDa (why is the actual band size different from the predicted?)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human kidney tissue labelling PPAP2A with ab58745 at 4-8µg/ml. Arrows indicate positively labelled epithelial cells of the renal tubule. Magnification: 400X.

参考文献

This product has been referenced in:
  • Evseenko D  et al. Lysophosphatidic acid mediates myeloid differentiation within the human bone marrow microenvironment. PLoS One 8:e63718 (2013). IHC-P ; Human . Read more (PubMed: 23696850) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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