Rabbit polyclonal to PP5
Mouse, Rat, Dog
Synthetic peptide derived from within residues 150 - 250 of Human PP5.
(Peptide available as ab27751.)
- This antibody gave a positive signal in HeLa, Jurkat and A431 whole cell lysates
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Concentration information loading...
Immunogen affinity purified
Immunizing Peptide (Blocking)
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
||Use a concentration of 1 - 5 µg/ml. Detects a band of approximately 59 kDa (predicted molecular weight: 57 kDa).Can be blocked with Human PP5 peptide (ab27751).
May play a role in the regulation of RNA biogenesis and/or mitosis. In vitro, dephosphorylates serine residues of skeletal muscle phosphorylase and histone H1.
Belongs to the PPP phosphatase family. PP-5 (PP-T) subfamily.
Contains 3 TPR repeats.
Nucleus. Cytoplasm. Predominantly nuclear. But also present in the cytoplasm.
Information by UniProt
- FLJ36922 antibody
- FLJ55954 antibody
- OTTHUMP00000165834 antibody
Western blot - Anti-PP5 antibody (ab34693)
All lanes :
Anti-PP5 antibody (ab34693) at 1 µg/mlLane 1 :
HeLa (Human epithelial carcinoma cell line) Whole Cell LysateLane 2 : Jurkat whole cell lysate (ab7899)
Lane 3 : A431 whole cell lysate (ab7909)
Lysates/proteins at 20 µg per lane.
All lanes : Goat polyclonal to Rabbit IgG (Alexa Fluor® 680) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size: 57 kDa
Observed band size: 59 kDa (why is the actual band size different from the predicted?)
Additional bands at: 75 kDa (possible cross reactivity)
ab34693 detects a band of approximately 59 kDa which corresponds in size to PP5. It also crossreacts with a band at 75 kDa in HeLa cell extract which we cannot identify.
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