製品の概要

  • 製品名PP2
  • 製品の詳細
    Src and RIP2 kinase inhibitor
  • 生理活性の詳細Inhibitor of the Src family of kinases. Inhibits Src and Lck kinase (IC50 values are 36 and 31 nM, respectively). Also shown to inhibit RIP2 and CK1δ (IC50 values are 19 and 41 nM, respectively).
  • 精製度> 98%

製品の特性

  • 体系名1-tert-Butyl-3-(4-chlorophenyl)-1H-pyrazolo[3,4-d]pyrimidin-4-amine
  • 分子量301.78
  • 構造式Chemical Structure
  • 分子式C15H16ClN5
  • CAS 番号172889-27-9
  • PubChem 登録番号4878
  • 保存方法Store at +4°C. Store under Desiccating conditions. The product can be stored for up to 12 months.
  • 溶解性Soluble in DMSO to 50 mM and in ethanol to 10 mM
  • 使用に関する注意

    Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20°C. Generally, these will be useable for up to one month. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.

    Need more advice on solubility, usage and handling? Please visit our frequently asked questions (FAQ) page for more details.

  • SMILES 線形表記Clc1ccc(cc1)c3nn(c2ncnc(N)c23)C(C)(C)C
  • 由来

    Synthetic

  • 研究分野

    アプリケーション

    Our Abpromise guarantee covers the use of ab120308 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    アプリケーション Abreviews 特記事項
    Functional Studies Use at an assay dependent concentration.

    PP2 画像

    • ab11512 staining E-Cadherin in SW480 cells treated with PP2 (ab120308), by ICC/IF. Increase in E-cadherin expression correlates with increased concentration of PP2, as described in literature.
      The cells were incubated at 37°C for 24h in media containing different concentrations of ab120308 (PP2) in DMSO, fixed with 4% formaldehyde for 10 minutes at room temperature and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab11512 (5 µg/ml) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 goat anti-rat polyclonal antibody (ab96971) at 1/250 dilution was used as the secondary antibody. Nuclei were counterstained with DAPI and are shown in blue.

    PP2 (ab120308) 使用論文

    This product has been referenced in:

    See 0 Publications for this product

    Product Wall

    There are currently no Abreviews or Questions for ab120308.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE, NOT FOR USE IN HUMANS"