製品の概要

  • 製品名Anti-PP11 antibody
    PP11 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to PP11
  • アプリケーション適用あり: ICC/IF, IHC-Pmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Cow
  • 免疫原

    Recombinant fragment, corresponding to amino acids 116-253 of Human PP11 (P21128).

  • ポジティブ・コントロール
    • Human placenta tissue.

製品の特性

関連製品

アプリケーション

Our Abpromise guarantee covers the use of ab121367 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF Use a concentration of 1 - 4 µg/ml.

Recommend PFA Fixation and Triton X-100 treatment

IHC-P 1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

ターゲット情報

  • 機能Endoribonuclease that cleaves single-stranded RNAs at uridylates and releases products that have 2'-3'-cyclic phosphate termini.
  • 組織特異性Placental-specific, but also associated with various malignant neoplasms.
  • 配列類似性Belongs to the ENDOU family.
    Contains 2 SMB (somatomedin-B) domains.
  • 翻訳後修飾It has been suggested that the active SMB domain may be permitted considerable disulfide bond heterogeneity or variability, thus two alternate disulfide patterns based on 3D structures are described with 1 disulfide bond conserved in both.
  • 細胞内局在Secreted.
  • Information by UniProt
  • 参照データベース
  • 別名
    • 22 serine protease antibody
    • 26 serine protease antibody
    • Endonuclease, polyU-specific antibody
    • endou antibody
    • ENDOU_HUMAN antibody
    • P11 antibody
    • Placental protein 11 antibody
    • Poly(U)-specific endoribonuclease antibody
    • PP11 antibody
    • Pp11r antibody
    • Protein endoU antibody
    • PRSS26 antibody
    • Uridylate-specific endoribonuclease antibody
    see all

Anti-PP11 antibody 画像

  • Immunofluorescent staining of Human cell line U-251MG shows positivity in nucleus but not nucleoli and cytoplasm. Recommended concentration of ab121367 1-4 µg/ml. Cells treated with PFA/Triton X-100.
  • Immunocytochemical immunofluorescence analysis of methanol-fixed human HEK293T cells, labelling PP11 with 121367 at a dilution of 1/100 incubated for 16 hours at 4°C in 1% BSA in PBS with 0.1% Tween20 diluent. Blocking was with 1% BSA incubated for 1 hour at 25°C. Secondary was a goat anti-rabbit polyclonal Rhodamine conjugate at 1/100.

    See Abreview

  • ab121367 at 1/250 dilution staining PP11 in Paraffin-emebedded Human placenta tissue by Immunohistochemistry.

Anti-PP11 antibody (ab121367) 使用論文

ab121367 has not yet been referenced specifically in any publications.

Product Wall

Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (HEK293T)
Permeabilization No
Specification HEK293T
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C
Fixative Methanol
Username

Mr. Chi-Cheng Hsieh

Verified customer

投稿 Nov 05 2015

Abreviews
Abcam has not validated the combination of species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (HEK293T)
Gel Running Conditions Reduced Denaturing
Loading amount 50 µg
Specification HEK293T
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Mr. Chi-Cheng Hsieh

Verified customer

投稿 Oct 30 2015

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"