The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 - 4 µg/ml.
Recommend PFA Fixation and Triton X-100 treatment
1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Endoribonuclease that cleaves single-stranded RNAs at uridylates and releases products that have 2'-3'-cyclic phosphate termini.
Placental-specific, but also associated with various malignant neoplasms.
Belongs to the ENDOU family. Contains 2 SMB (somatomedin-B) domains.
It has been suggested that the active SMB domain may be permitted considerable disulfide bond heterogeneity or variability, thus two alternate disulfide patterns based on 3D structures are described with 1 disulfide bond conserved in both.
Immunofluorescent staining of Human cell line U-251MG shows positivity in nucleus but not nucleoli and cytoplasm. Recommended concentration of ab121367 1-4 µg/ml. Cells treated with PFA/Triton X-100.
Immunocytochemistry/ Immunofluorescence - Anti-PP11 antibody (ab121367)Image is courtesy of an AbReview submitted by Mr Chi-Cheng Hsieh.
Immunocytochemical immunofluorescence analysis of methanol-fixed human HEK293T cells, labelling PP11 with 121367 at a dilution of 1/100 incubated for 16 hours at 4°C in 1% BSA in PBS with 0.1% Tween20 diluent. Blocking was with 1% BSA incubated for 1 hour at 25°C. Secondary was a goat anti-rabbit polyclonal Rhodamine conjugate at 1/100.