製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to POU3F3
  • アプリケーション
    適用あり: WBmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat
  • 免疫原

    Synthetic peptide corresponding to a region within the N terminal amino acids 36-85 (GGGGGGGGAG GGGGGMQPGS AAVTSGAYRG DPSSVKMVQS DFMQGAMAAS) of Human POU3F3 (NP_006227).

  • ポジティブ・コントロール
    • OVCAR-3 cell lysate

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab90727 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 50 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

ターゲット情報

  • 機能
    Transcription factor that acts synergistically with SOX11 and SOX4. Plays a role in neuronal development. Is implicated in an enhancer activity at the embryonic met-mesencephalic junction; the enhancer element contains the octamer motif (5'-ATTTGCAT-3').
  • 組織特異性
    Brain.
  • 配列類似性
    Belongs to the POU transcription factor family. Class-3 subfamily.
    Contains 1 homeobox DNA-binding domain.
    Contains 1 POU-specific domain.
  • 細胞内局在
    Nucleus.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Brain 1 antibody
    • Brain specific homeobox/POU domain protein 1 antibody
    • Brain-1 antibody
    • Brain-specific homeobox/POU domain protein 1 antibody
    • Brain-specific homeobox/POU domain protein 1.1 antibody
    • Brain1 antibody
    • BRN 1 antibody
    • Brn-1 antibody
    • BRN1 antibody
    • Brn1, mouse, homolog of antibody
    • class 3 antibody
    • Class III POU domain protein taichi antibody
    • Oct-8 antibody
    • Octamer-binding protein 8 antibody
    • Octamer-binding transcription factor 8 antibody
    • OTF 8 antibody
    • OTF-8 antibody
    • OTF8 antibody
    • PO3 F3 antibody
    • PO3F 3 antibody
    • PO3F3 antibody
    • PO3F3_HUMAN antibody
    • POU class 3 homeobox 3 antibody
    • POU domain antibody
    • POU domain protein 12 antibody
    • POU domain, class 3, transcription factor 3 antibody
    • POU domain, class 3, transcription factor 3-A antibody
    • pou12 antibody
    • POU3 F3 antibody
    • POU3F 3 antibody
    • Pou3f3 antibody
    • RHS 1 antibody
    • Rhs 2 antibody
    • RHS1 antibody
    • Rhs2 antibody
    • Skin 1 antibody
    • Skin1 antibody
    • tai-ji antibody
    • transcription factor 3 antibody
    • zp12 antibody
    • zp12pou antibody
    see all

Anti-POU3F3 antibody 画像



  • Predicted band size : 50 kDa
    Western blot analysis of Human fetal lung tissue lysate labeling POU3F3 with ab90727 at 1.0µg/ml.
  • Anti-POU3F3 antibody (ab90727) at 1 µg/ml (in 5% skim milk / PBS buffer) + OVCAR-3 cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 50 kDa
    Gel concentration: 12%

プロトコール

Anti-POU3F3 antibody (ab90727) 使用論文

ab90727 has not yet been referenced specifically in any publications.

Product Wall

Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (brain)
Permeabilization
Yes - triton X-100
Specification
brain
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Fixative
Paraformaldehyde
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投稿 Jun 29 2017

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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