The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 0.1 - 0.3 µg/ml. Detects a band of approximately 45 kDa (predicted molecular weight: 47 kDa).
Transcription factor that binds preferentially to the recognition sequence which consists of two distinct half-sites, ('GCAT') and ('TAAT'), separated by a nonconserved spacer region of 0, 2, or 3 nucleotides. Positively regulates the genes under the control of corticotropin-releasing hormone (CRH) and CRH II promoters.
Expressed specifically in the neuroectodermal cell lineage.
Belongs to the POU transcription factor family. Class-3 subfamily. Contains 1 homeobox DNA-binding domain. Contains 1 POU-specific domain.