製品の概要

  • 製品名
  • 製品の詳細
    Goat polyclonal to POU2F3
  • 由来種
    Goat
  • アプリケーション
    適用あり: WBmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Rat
  • 免疫原

    Synthetic peptide, corresponding to internal sequence amino acids 161-175 (

    EPHLEASQHLPVPKH

    ) of Human POU2F3 (NP_055167.2).

  • ポジティブ・コントロール
    • HEK293 and HepG2 cell lines.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab101724 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 - 3 µg/ml. Predicted molecular weight: 47 kDa.

ターゲット情報

  • 機能
    Transcription factor that binds to the octamer motif (5'-ATTTGCAT-3'). Regulated the expression of a number of genes such as SPRR2A or placental lactogen.
  • 組織特異性
    Specifically expressed in epidermis and cultured keratinocytes.
  • 配列類似性
    Belongs to the POU transcription factor family. Class-2 subfamily.
    Contains 1 homeobox DNA-binding domain.
    Contains 1 POU-specific domain.
  • 細胞内局在
    Nucleus.
  • Information by UniProt
  • 参照データベース
  • 別名
    • class 2 antibody
    • Epoc 1 antibody
    • Epoc1 antibody
    • HGNC19864 antibody
    • Oct 11 antibody
    • Oct-11 antibody
    • OCT11 antibody
    • Octamer binding transcription factor 11 antibody
    • Octamer-binding protein 11 antibody
    • Octamer-binding transcription factor 11 antibody
    • OTF-11 antibody
    • PLA 1 antibody
    • PLA1 antibody
    • PLA1 protein antibody
    • PO2F3_HUMAN antibody
    • POU class 2 homeobox 3 antibody
    • POU domain antibody
    • POU domain transcription factor OCT11a antibody
    • POU domain, class 2, transcription factor 3 antibody
    • POU transcription factor antibody
    • POU2F3 antibody
    • Skn 1a antibody
    • transcription factor 3 antibody
    • Transcription factor PLA-1 antibody
    • Transcription factor Skn-1 antibody
    • Transcription factor Skn1 antibody
    see all

画像

  • Anti-POU2F3 antibody (ab101724) at 2 µg/ml + HEK293 lysate in RIPA buffer at 35 µg

    Predicted band size: 47 kDa



    Incubation of ab101724 was 1 hour. Detected by chemiluminescence.

プロトコール

参考文献

ab101724 has not yet been referenced specifically in any publications.

レビューと Q&A

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (skin)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: citric buffer, EDTA
Permeabilization
No
Specification
skin
Blocking step
(agent) for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 20°C
Fixative
Paraformaldehyde
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投稿 Mar 12 2018

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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