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RabMAb

Anti-POT1 抗体 [EPR6319] (ab124784)

製品の概要

  • 製品名
    Anti-POT1 antibody [EPR6319]
    POT1 一次抗体 製品一覧
  • 製品の詳細
    Rabbit monoclonal [EPR6319] to POT1
  • アプリケーション
    適用あり: WBmore details
    適用なし: Flow Cyt,ICC/IF,IHC-P or IP
  • 種交差性
    交差種: Human
  • 免疫原

    Synthetic peptide corresponding to residues in Human POT1.

  • ポジティブ・コントロール
    • K562, HepG2, 293T, and HeLa cell lysates.
  • 特記事項

    This product is a recombinant rabbit monoclonal antibody.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab124784 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/1000 - 1/10000. Predicted molecular weight: 71 kDa.
  • 追加情報
    Is unsuitable for Flow Cyt,ICC/IF,IHC-P or IP.
  • ターゲット情報

    • 機能
      Component of the telomerase ribonucleoprotein (RNP) complex that is essential for the replication of chromosome termini. Is a component of the double-stranded telomeric DNA-binding TRF1 complex which is involved in the regulation of telomere length by cis-inhibition of telomerase. Also acts as a single-stranded telomeric DNA-binding protein and thus may act as a downstream effector of the TRF1 complex and may transduce information about telomere maintenance and/or length to the telomere terminus. Component of the shelterin complex (telosome) that is involved in the regulation of telomere length and protection. Shelterin associates with arrays of double-stranded TTAGGG repeats added by telomerase and protects chromosome ends; without its protective activity, telomeres are no longer hidden from the DNA damage surveillance and chromosome ends are inappropriately processed by DNA repair pathways. Binds to two or more telomeric single-stranded 5'-TTAGGG-3' repeats (G-strand) and with high specificity to a minimal telomeric single-stranded 5'-TAGGGTTAG-3' sequence. Binds telomeric single-stranded sequences internally or at proximity of a 3'-end. Its activity is TERT dependent but it does not increase TERT activity by itself. In contrast, the ACD-POT1 heterodimer enhances telomer elongation by increasing telomerase processivity.
    • 組織特異性
      Ubiquitous.
    • 配列類似性
      Belongs to the telombin family.
    • 細胞内局在
      Nucleus. Chromosome > telomere. Colocalizes with telomeric DNA.
    • Information by UniProt
    • 参照データベース
    • 別名
      • DKFZP586D211 antibody
      • hPot 1 antibody
      • hPot1 antibody
      • POT 1 antibody
      • POT1 antibody
      • POT1 like telomere end-binding protein antibody
      • POT1 protection of telomeres 1 homolog antibody
      • POT1-like telomere end-binding protein antibody
      • POTE1_HUMAN antibody
      • Protection of telomeres 1 antibody
      • Protection of telomeres 1 homolog (S. pombe) antibody
      • Protection of telomeres protein 1 antibody
      see all

    画像

    • All lanes : Anti-POT1 antibody [EPR6319] (ab124784) at 1/1000 dilution

      Lane 1 : K562 cell lysate
      Lane 2 : HepG2 cell lysate
      Lane 3 : 293T cell lysate
      Lane 4 : HeLa cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      Standard HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size : 71 kDa
    • Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD

    プロトコール

    参考文献

    This product has been referenced in:
    • Feng X  et al. CTC1-mediated C-strand fill-in is an essential step in telomere length maintenance. Nucleic Acids Res 45:4281-4293 (2017). WB ; Human . Read more (PubMed: 28334750) »
    • Takai H  et al. A POT1 mutation implicates defective telomere end fill-in and telomere truncations in Coats plus. Genes Dev 30:812-26 (2016). Read more (PubMed: 27013236) »

    See all 3 Publications for this product

    レビューと Q&A

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (LCL cells)
    Loading amount
    30 µg
    Specification
    LCL cells
    Gel Running Conditions
    Reduced Denaturing (10% SDS)
    Blocking step
    Milk as blocking agent for 18 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
    Username

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    Verified customer

    投稿 Nov 14 2012

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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