製品の概要

  • 製品名Anti-PON3 antibody [5G11]
    PON3 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [5G11] to PON3
  • アプリケーション適用あり: IHC-P, WB, ICC/IFmore details
  • 種交差性
    交差種: Human
    非交差種: Mouse, Rat
  • 免疫原

    Recombinant human protein purified from E.coli (His-PON3)

  • ポジティブ・コントロール
    • HepG2 and Hep3B cell lysates

製品の特性

関連製品

アプリケーション

Our Abpromise guarantee covers the use of ab71994 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/2000. Detects a band of approximately 40 kDa (predicted molecular weight: 40 kDa).
ICC/IF Use a concentration of 1 µg/ml.

ターゲット情報

  • 機能Has low activity towards the organophosphate paraxon and aromatic carboxylic acid esters. Rapidly hydrolyzes lactones such as statin prodrugs (e.g. lovastatin). Hydrolyzes aromatic lactones and 5- or 6-member ring lactones with aliphatic substituents but not simple lactones or those with polar substituents.
  • 配列類似性Belongs to the paraoxonase family.
  • 翻訳後修飾The signal sequence is not cleaved.
  • 細胞内局在Secreted > extracellular space.
  • Information by UniProt
  • 参照データベース
  • 別名
    • 2810004E20 antibody
    • AI786302 antibody
    • MGC95026 antibody
    • Paraoxonase 3 antibody
    • Pon3 antibody
    • PON3_HUMAN antibody
    • Serum paraoxonase/lactonase 3 antibody
    see all

Anti-PON3 antibody [5G11] 画像

  • All lanes : Anti-PON3 antibody [5G11] (ab71994) at 1/2000 dilution

    Lane 1 : HepG2 cell lysate
    Lane 2 : Hep3B cell lysate


    Predicted band size : 40 kDa
    Observed band size : 40 kDa
  • IHC image of ab71994 staining in human normal liver formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab71994, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

Anti-PON3 antibody [5G11] (ab71994) 使用論文

ab71994 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"