製品の概要

  • 製品名Anti-POMC antibody
    POMC 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to POMC
  • アプリケーション適用あり: WB, IHC-Pmore details
  • 種交差性
    交差種: Mouse, Rat
    交差が予測される動物種: Human, Chimpanzee, Macaque Monkey, Gorilla, Chinese Hamster, Orangutan
  • 免疫原

    Synthetic peptide corresponding to Rat POMC aa 100-200 conjugated to Keyhole Limpet Haemocyanin (KLH).
    Database link: P01194

  • ポジティブ・コントロール
    • This antibody gave a positive signal in PC12 whole cell lysate as well as Mouse and Rat Brain tissue lysates. This antibody gave a positive result in IHC in the following frozen tissue: Rat Brain (Arcuate Nucleus of the Hypothalamus).

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab94446 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 µg/ml. Detects a band of approximately 26 kDa (predicted molecular weight: 26 kDa).
IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

ターゲット情報

  • Information by UniProt
  • 参照データベース
  • 別名
    • ACTH antibody
    • Adrenocorticotropic hormone antibody
    • Adrenocorticotropin antibody
    • alpha melanocyte stimulating hormone antibody
    • Alpha MSH antibody
    • Alpha-MSH antibody
    • beta endorphin antibody
    • Beta LPH antibody
    • beta melanocyte stimulating hormone antibody
    • Beta MSH antibody
    • Beta-LPH antibody
    • Beta-MSH antibody
    • CLIP antibody
    • COLI_HUMAN antibody
    • Corticotropin like intermediary peptide antibody
    • Corticotropin lipotropin antibody
    • Corticotropin-lipotropin antibody
    • Gamma MSH antibody
    • Gamma-LPH antibody
    • Gamma-MSH antibody
    • Lipotropin beta antibody
    • Lipotropin gamma antibody
    • LPH antibody
    • Melanotropin alpha antibody
    • Melanotropin antibody
    • Melanotropin beta antibody
    • Melanotropin gamma antibody
    • met enkephalin antibody
    • Met-enkephalin antibody
    • MSH antibody
    • NPP antibody
    • POC antibody
    • POMC antibody
    • pro ACTH endorphin antibody
    • Proopiomelanocortin antibody
    • Proopiomelanocortin preproprotein antibody
    see all

Anti-POMC antibody 画像

  • IHC image of POMC staining in Rat Brain (Arcuate Nucleus of the Hypothalamus) formalin fixed frozen tissue section, performed on a Leica Bond™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab94446, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

  • All lanes : Anti-POMC antibody (ab94446) at 1 µg/ml

    Lane 1 : Brain (Rat) Tissue Lysate
    Lane 2 : Brain (Mouse) Tissue Lysate
    Lane 3 : PC12 (Rat adrenal pheochromocytoma cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 26 kDa
    Observed band size : 26 kDa


    Exposure time : 30 seconds

    This blot was produced using a 4-12% Bis-tris gel under the MES buffer system. The gel was run at 200V for 35 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Bovine Serum Albumin before being incubated with ab94446 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.

Anti-POMC antibody (ab94446) 使用論文

ab94446 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"