The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
70 - 90% by HPLC. purified
ab85007 was expressed by fermentation of Bacillus polymyxa. The peptide was subsequently purified by HPLC.
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Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Reconstitute the vial with distilled or deionized water, volume depending on the concentration to be used.
After reconstitution store stock solution in aliquots at -20°C.
The peptide antibiotic Polymyxin B (PMB) binds to bacterial endotoxin
(lipopolysaccharide, LPS). The interaction of PMB with LPS involves ionic
forces between amino groups in PMB and negatively charged phosphate
and carboxyl groups in the lipid A-Kdo region. PMB has relevance for
endotoxin research in at least two ways: first, PMB reacts with LPS of
many species regardless of varied serospecificity, and thus it can be used
as a general probe for measuring or detecting LPS or lipid A. Second,
binding of PMB to LPS may result in neutralization of the detrimental
effects of LPS either in vitro or in vivo.
has not yet been referenced specifically in any publications.