Anti-polyHistidine 抗体 [HIS-1] (HRP) (ab49781)

製品の概要

  • 製品名
    Anti-polyHistidine antibody [HIS-1] (HRP)
    polyHistidine 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [HIS-1] to polyHistidine (HRP)
  • 標識
    HRP
  • 特異性
    This antibody recognizes native as well as denatured, reduced forms of synthetic polyhistidine or polyhistidine tagged fusion proteins. The product is reactive with fusion protein expressed by prokaryotic pET, pRSET and pTrc expression vectors.
  • アプリケーション
    適用あり: ELISA, Dot blot, WBmore details
  • 免疫原

    Recombinant polyHistidine tagged fusion protein.

  • ポジティブ・コントロール
    • Bacteria lysates expressing a recombinant Histidine tagged fusion protein.
  • 特記事項

    Monoclonal antibody reacting specifically with polyHistidine may be useful in various immunotechniques, to identify the expression of a polyhistidine fusion protein in bacteria, bacterial lysates or cells and tissues transfected with a polyHistidine tagged fusion protein expressing vectors.

    The conjugate was prepared by conjugation of HRP to Mouse monoclonal anti-polyHistidine purified from ascites fluid of the HIS-1 hybridoma. Molar ratio Ab/Enzyme: 0.8 to 1.5 Enzyme activity: at least 400 U/ml

法規制情報

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Store at +4°C.
  • バッファー
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 0.0268% PBS, 1% BSA
  • Concentration information loading...
  • 精製度
    IgG fraction
  • 一次抗体 備考
    Monoclonal antibody reacting specifically with polyHistidine may be useful in various immunotechniques, to identify the expression of a polyhistidine fusion protein in bacteria, bacterial lysates or cells and tissues transfected with a polyHistidine tagged fusion protein expressing vectors.
  • ポリ/モノ
    モノクローナル
  • クローン名
    HIS-1
  • アイソタイプ
    IgG2a
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab49781 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ELISA Use at an assay dependent concentration.
Dot blot 1/2000.
WB 1/2000.

ターゲット情報

  • 関連性
    Recombinant DNA technology enables the attachment of genes of interest to specific sequences or genes that can provide 'affinity handles' (tags) designed to enable the selective identification of the protein of interest. These sequences of tails or tags are genetically engineered away from the protein active site, by insertion at the N or C terminus. It has been reported that the addition of a consecutive Histidine amino acid residue tail creates a stable fusion product that does not appear to interfere with the bioactivity of the protein or with the biodistribution of the Histidine tagged product.

プロトコール

Anti-polyHistidine antibody [HIS-1] (HRP) (ab49781) 使用論文

ab49781 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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