Anti-Polyethylene glycol 抗体 [26A04] - BSA and Azide free (ab94764)


  • 製品名Anti-Polyethylene glycol antibody [26A04] - BSA and Azide free
    Polyethylene glycol 一次抗体 製品一覧
  • 製品の詳細
    Rat monoclonal [26A04] to Polyethylene glycol - BSA and Azide free
  • 特異性ab94764 recognizes linear 20 kDa PEG, linear 5 kDa PEG, linear 600 Da PEG and 10 kDa branched PEG. It also binds to BSA conjugated PEG by western blot.
  • アプリケーション適用あり: IHC-P, WB, Indirect ELISAmore details
  • 免疫原

    5 kDa PEG conjugated to KLH by a MBS linker.

  • ポジティブ・コントロール
    • PEGylated protein
  • 特記事項

    Tween 20 usage: Tween 20 is a detergent that can interfere with ELISA and potentially other applications (often used in antibody dilution buffers, such as TBST) when using anti-PEG products. This is also the same case for other polyoxyethylene detergents. Therefore, it is recommended to avoid Tween 20 and other polyoxyethylene detergents
    when performing ELISA using anti-PEG products.


    Learn more about PEG RabMAb primary antibodies and kit - PEG RabMAb Product Portal Page


  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • バッファーConstituent: Tris buffered saline
  • Concentration information loading...
  • 特記事項(精製)Ceramic hydroxyapatite (CHT) chromatography from in vitro production.
  • ポリ/モノモノクローナル
  • クローン名26A04
  • アイソタイプIgM
  • 研究分野


Our Abpromise guarantee covers the use of ab94764 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P Use a concentration of 8 µg/ml.
WB Use at an assay dependent dilution. Predicted molecular weight: 1 kDa.
Indirect ELISA Use a concentration of 2 µg/ml.


  • 別名
    • PEG antibody

Anti-Polyethylene glycol antibody [26A04] - BSA and Azide free 画像

  • Indirect ELISA analysis of a PEG panel using ab94764.
  • Anti-Polyethylene glycol antibody [26A04] - BSA and Azide free (ab94764) + PEGylated protein

    Predicted band size : 1 kDa

Anti-Polyethylene glycol antibody [26A04] - BSA and Azide free (ab94764) 使用論文

ab94764 has not yet been referenced specifically in any publications.

Product Wall

To avoid Tween20 and other PEG-containing detergents, I recommend antigen retrieval buffers (100x Citrate Buffer pH 6.0, 50mL) or (10...

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Thank you for your enquiry and your interest.

I can confirm that we have determined that the specificity of the antibody (ab94764: Anti-Polyethylene glycol antibody) is to the linear backbone of PEG and that it will not recognize t...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Mouse Tissue sections (Atheroma on aorta of ApoE-KO mice)
Specification Atheroma on aorta of ApoE-KO mice
Fixative Paraformaldehyde
Antigen retrieval step None
Permeabilization Yes - baths in 0.1% Tween-20 diluted in PBS
Blocking step Protein-Free Blocking Buffer, Pierce, Cat N° 37570 as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 21°C

Abcam user community

Verified customer

投稿 Jan 12 2012

Thank you very much for your valuable feedback. We would be very grateful if you could submit an Abreview about this product via the online product datasheet. We always encourage customers to send their results back to us, whether positive or negative,...

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Thank you for your reply. If the secondary antibody is directed against the heavy and light chains (H+L) of rat IgG, you will still be able to observe specific signal because the light chains are conserved between isotypes. If the secondary antibody is...

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