製品の概要

  • 製品名
    Anti-Polycystin 1 antibody [7e12]
    Polycystin 1 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [7e12] to Polycystin 1
  • アプリケーション
    適用あり: IHC-P, Flow Cyt, ICC/IFmore details
    適用なし: WB
  • 種交差性
    交差種: Mouse, Rat, Dog, Human
  • 免疫原

    Bacterially expressed fusion protein from the N-terminal leucine rich domain of human Polycystin 1.

  • エピトープ
    This antibody was produced to the flank-leucine rich repeat-flank region (24-180aa).
  • ポジティブ・コントロール
    • This antibody gave a positive signal when used to probe a Human Liver, bone marrow, and KidneyTissue section (IHC).
  • 特記事項

    This antibody clone is manufactured by Abcam.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab74115 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P Use a concentration of 5 µg/ml.
Flow Cyt Use 1-2µg for 106 cells. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
ICC/IF Use a concentration of 5 µg/ml.
  • 追加情報
    Is unsuitable for WB.
  • ターゲット情報

    • 機能
      May be an ion-channel regulator. PKD1 and PKD2 may function through a common signaling pathway that is necessary for normal tubulogenesis. Involved in adhesive protein-protein and protein-carbohydrate interactions.
    • 関連疾患
      Defects in PKD1 are the cause of polycystic kidney disease autosomal dominant type 1 (ADPKD1) [MIM:173900]. ADPKD is characterized by progressive formation and enlargement of cysts in both kidneys, typically leading to end-stage renal disease in adult life. Cysts also occurs in the liver and other organs. Its prevalence is estimated at about 1/1000.
    • 配列類似性
      Belongs to the polycystin family.
      Contains 1 C-type lectin domain.
      Contains 1 GPS domain.
      Contains 1 LDL-receptor class A domain.
      Contains 2 LRR (leucine-rich) repeats.
      Contains 1 LRRCT domain.
      Contains 1 LRRNT domain.
      Contains 17 PKD domains.
      Contains 1 PLAT domain.
      Contains 1 REJ domain.
      Contains 1 WSC domain.
    • ドメイン
      The LDL-receptor class A domain is atypical; the potential calcium-binding site is missing.
    • 細胞内局在
      Membrane.
    • Information by UniProt
    • 参照データベース
    • 別名
      • Autosomal dominant polycystic kidney disease 1 protein antibody
      • Autosomal dominant polycystic kidney disease protein 1 antibody
      • nPKC-D1 antibody
      • nPKC-mu antibody
      • OTTHUMP00000208669 antibody
      • OTTHUMP00000208670 antibody
      • PBP antibody
      • Pc-1 antibody
      • PKD antibody
      • Pkd1 antibody
      • PKD1_HUMAN antibody
      • Polycystic Kidney Disease 1 antibody
      • polycystic kidney disease-associated protein antibody
      • Polycystin 1 Precursor antibody
      • Polycystin-1 antibody
      • Protein kinase C mu type antibody
      • Protein kinase D antibody
      • Serine/threonine-protein kinase D1 antibody
      • transient receptor potential cation channel, subfamily P, member 1 antibody
      • TRPP1 antibody
      see all

    Anti-Polycystin 1 antibody [7e12] 画像

    • IHC image of Polycystin 1 staining in a formalin fixed, paraffin embedded normal human kidney tissue section*, performed on a Leica Bond™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab74115, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

       

      For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

      *Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre

    • IHC image of Polycystin 1 staining in Human Normal Liver FFPE section, performed on a BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab74115, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX
    • IHC image of Polycystin 1 staining in Normal Human Bone Marrow formalin fixed paraffin embedded tissue section*, performed on a Leica Bond™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab74115, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

       

      For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

      *Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre

    • Overlay histogram showing HEK293 cells stained with ab74115 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab74115, 2µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells ) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HEK293 cells fixed with 80% methanol (5 min)/permeabilized in 0.1% PBS-Tween used under the same conditions.

    Anti-Polycystin 1 antibody [7e12] (ab74115) 使用論文

    This product has been referenced in:
    • Lu H  et al. Mutations in DZIP1L, which encodes a ciliary-transition-zone protein, cause autosomal recessive polycystic kidney disease. Nat Genet 49:1025-1034 (2017). Read more (PubMed: 28530676) »
    • Aguiari G  et al. Novel role for polycystin-1 in modulating cell proliferation through calcium oscillations in kidney cells. Cell Prolif 41:554-73 (2008). Read more (PubMed: 18422703) »

    See all 9 Publications for this product

    Product Wall

    Abcam has not validated the combination of species/application used in this Abreview.
    Application
    Western blot
    Sample
    Dog Cell lysate - whole cell (kidney nephron)
    Gel Running Conditions
    Non-reduced Non-Denaturing (Native) (6)
    Loading amount
    300 µg
    Specification
    kidney nephron
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 4°C
    Username

    Herr Dr. Andre Kraus

    Verified customer

    投稿 Mar 31 2017

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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